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PEMBENTUKAN TMAO

PEMBENTUKAN TMAO. YUNITA EKA PUSPITASARI, S.Pi , MP FAKULTAS PERIKANAN DAN ILMU KELAUTAN UNIVERSITAS BRAWIJAYA MALANG. Mekanisme Pembentukan TMAO. Oksidasi Enzimatik TMAO  hasil oksidasi TMA Katalis enzim  TMA monooksigenase

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PEMBENTUKAN TMAO

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  1. PEMBENTUKAN TMAO YUNITA EKA PUSPITASARI, S.Pi, MP FAKULTAS PERIKANAN DAN ILMU KELAUTAN UNIVERSITAS BRAWIJAYA MALANG

  2. MekanismePembentukan TMAO • OksidasiEnzimatik • TMAO  hasiloksidasi TMA • Katalisenzim  TMA monooksigenase • (CH3)3N + NADPH2 + 02  (CH3)3NO + NADP + H20 • Choline prekursor TMA

  3. MekanismePembentukan TMAO • Oksidasi Non enzimatik • Jaluroksidasi TMAO : • O2 berikatandengan TMA  TMAO • Dimetilasi TMA0  DMA +FA • Oksidasi N-dimetilasimenghasilkan DMA+FA secaralangsungdari TMA (TMA dehidrogenase)

  4. MEKANISME PEMBENTUKAN TMAO TMA N-Oxide C-assimilation by ribose phospate or serine pathways H20 2H + 02 TMA H20 Formaldehyde 2H H20 2H DMA 2H + 02 Formate H20 Methylamine 2H H20 CO2 Assimilation Ammonia 2H

  5. Reduksi TMAO • Jenisenzim reduksi TMAO menjadi TMA  DMA  FA • Enzim endogen padaikan • Enzimeksogendihasilkanbakteri • Aktivitasenzimberperandalampembusukanikan

  6. Bakteri • Bakteri yang mampumereduksi TMAO  TMA • Enterobacteriaceae • Escherichia coli, Achromobacter, Micrococcus, Flavobacterium • Pseudomonas, Clostridium, Alcaligenes, Bacillus spp.

  7. PengaruhPenyimpananDingin TMAO • TMA-dihasilkandariAchromobactersmenurundari 60%  30 % setelah 13 penyimpanandingin • Diskusi…..

  8. PUSTAKA Hebarda, C.E. G.J. Fillick and R.E. Martin. Occurance and Significance of TMAO and Its Derivataves in Fish and Shellfish. AVI Publishing Company Regenstin, J.M., M. A. Schlosser, A. Samson and M. Fey. Chemical changes of TMAO During Fresh and Frozen Storage Fish. AVI Publishing Company STR~MA,. R. 1971. Bakterielleomsetninger I anaerobtsilde-ekstrakt. Lic. techn. thesis, Norwegian Institute of Technology, University of Trondheim, Norway Seibel, B.A and Patrick J. Walsh. 2002. Trimethylamine oxide accumulation in marine animals: relationship to acylglycerol storage. The Journal of Experimental Biology 205, 297–306

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