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DNA replication

DNA replication. Understand the basic rules governing DNA replication Introduce proteins that are typically involved in generalised replication. Reference : Any of the recommended texts Optional Nature (2003) vol 421,pp431-435 http://www.bath.ac.uk/bio-sci/cbt/.

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DNA replication

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  1. DNA replication Understand the basic rules governing DNA replication Introduce proteins that are typically involved in generalised replication Reference: Any of the recommended texts Optional Nature (2003) vol 421,pp431-435 http://www.bath.ac.uk/bio-sci/cbt/ http://www.dnai.org/lesson/go/2166/1973

  2. `It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material’ Watson & Crick Nature (1953) Original drawing by Francis Crick

  3. Four requirements for DNA to be genetic material Must carry information • Cracking the genetic code Must replicate • DNA replication Must allow for information to change • Mutation Must govern the expression of the phenotype • Gene function

  4. DNA stores information in the sequence of its bases • Much of DNA’s sequence-specific information is accessible only when the double helix is unwound • Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. • Proteins can either bind to a DNA sequence, or initiate the copying of it. • Some genetic information is accessible even in intact, double-stranded DNA molecules • Some proteins recognize the base sequence of DNA without unwinding it (One example is a restriction enzyme).

  5. DNA replication occurs with great fidelity Somatic cell DNA stability and reproductive-cell DNA stability are essential. Why? Identity Pan troglodytes 99% sequence identity Homo sapiens sapiens 99.9% sequence identity Genetic diseases

  6. DNA Replication Process of duplication of the entire genome prior to cell division Biological significance • extreme accuracy of DNA replication is necessary in order to preserve the integrity of the genome in successive generations • In eukaryotes , replication only occurs during the S phase of the cell cycle. • Replication rate in eukaryotes is slower resulting in a higher fidelity/accuracy of replication in eukaryotes

  7. Basic rules of replication • Semi-conservative • Starts at the ‘origin’ • Synthesis always in the 5-3’ direction • Can be uni or bidirectional • Semi-discontinuous • RNA primers required

  8. DNA replication 3 possible models

  9. Semi-conservative replication: One strand of duplex passed on unchanged to each of the daughter cells. This 'conserved' strand acts as a template for the synthesis of a new, complementary strand by the enzyme DNA polymerase

  10. How do we know that DNA replication is semiconservative? Meselson-Stahl experiments

  11. B) Starts at origin Initiator proteins identify specific base sequences on DNA called sites of origin Prokaryotes – single origin site E.gE.coli - oriC Eukaryotes – multiple sites of origin (replicator) E.g. yeast - ARS (autonomously replicating sequences) Prokaryotes Eukaryotes

  12. In what direction does DNA replication occur? C) Synthesis is ALWAYS in the 5’-3’ direction What happens if a base mismatch occurs? Where does energy for addition of nucleotide come from?

  13. Why does DNA replication only occur in the 5’ to 3’ direction? Should be PPP here

  14. D) Uni or bidirectional • Replication forks move in one or opposite directions

  15. E) Semi-discontinuous replication Anti parallel strands replicated simultaneously • Leading strand synthesis continuously in 5’– 3’ • Lagging strand synthesis in fragments in 5’-3’

  16. Semi-discontinuous replication New strand synthesis always in the 5’-3’ direction

  17. F) RNA primers required

  18. Core proteins at the replication fork - Prevents torsion by DNA breaks - separates 2 strands - RNA primer synthesis - prevent reannealing of single strands - synthesis of new strand - stabilises polymerase - seals nick via phosphodiester linkage Topoisomerases Helicases Primase Single strand binding proteins DNA polymerase Tethering protein DNA ligase

  19. The mechanism of DNA replication Arthur Kornberg, a Nobel prize winner and other biochemists deduced steps of replication • Initiation • Proteins bind to DNA and open up double helix • Prepare DNA for complementary base pairing • Elongation • Proteins connect the correct sequences of nucleotides into a continuous new strand of DNA • Termination • Proteins release the replication complex

  20. The mechanism of DNA replication http://www.thelifewire.com Life: 7th ed - Chapter 11

  21. Core proteins at the replication fork Nature (2003) vol 421,pp431-435 Figure in ‘Big’ Alberts too

  22. What kind of enzyme synthesizes the new DNA strand? • RNA polymerase • DNA Polymerase • Primase • Helicase • Topoisomerase

  23. Eukaryotic chromosomes have multiple origins of replication • True • False

  24. In what direction is the newly synthesized DNA produced? • 5'-3' • 3'-5' • In the direction of the major groove • Both 5'-3' and 3'-5' depending on which strand is being replicated

  25. Nucleotides are always added to the growing DNA strand at the 3’ end, at which the DNA has a free ______ on the 3’ carbon of its terminal deoxyribose. • Phosphate group • Hydroxyl group • Nitrogen base • Methyl group

  26. The E. coli chromosome has 4.7x106 bp; a bi-directional replication fork progresses at about 1000 nucleotides/sec. Therefore, the minimum time required to complete replication is • 12 min. • 24 min. • 39 min • 78 min • 120 min

  27. What is the sequence (1 to 6) in which these proteins function during DNA replication • ____ RNA primase • ____ DNA ligase • ____ DNA polymerase • ____ Topoisomerase • ____ DNA helicase • ____ tethering proteins

  28. Why is an RNA primer necessary for DNA replication? • The RNA primer is necessary for the activity of DNA ligase. • The RNA primer creates the 5’ and 3’ ends of the strand. • DNA polymerase can only add nucleotides to RNA molecules. • DNA polymerase can only add nucleotides to an existing strand

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