1 / 22

About OMICS Group

About OMICS Group.

reina
Télécharger la présentation

About OMICS Group

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology ‘Open Access’, OMICS Group publishes 400 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 300 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.

  2. About OMICS Group Conferences OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Pharma scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.

  3. Y.ADIGUZEL, 12.08.2014 Progress on theStudies onVisual Detection and Surface Modification Testingof Glass MicrofiberFilter Based Biosensor YekbunADIGUZELa,Haluk KULAHb,c aDepartmentofBiophysics,SchoolofMedicine,IstanbulKemerburgazUniversity, MahmutbeyDilmenlerCaddesi,No:26, 34217Bagcilar,Istanbul, Turkey;email: yekbun.adiguzel@kemerburgaz.edu.tr bMETU-MEMSResearchandApplicationCenter,MiddleEastTechnicalUniversity (METU),Ankara,Turkey;email:kulah@metu.edu.tr cElectricalandElectronicsEngineeringDepartment,MiddleEastTechnicalUniversity (METU),UniversitelerMah.,DumlupinarBulv. No:1,06800Cankaya,Ankara,Turkey

  4. Y.ADIGUZEL, 12.08.2014 Summary of PreviousWork ►Surfaceofglassmicrofiberpaperwasmodifiedwith3-aminopropyltriethoxysilane(APTES)andwassuggestedtobeusedasabiosensorinthissurface-modifiedform. ►Asanapplication,visualdetectionofthesurface-immobilizedyeastcellswasachievedbyGramstainingandshowntobeincreasingbymodificationwithAPTES.(This’ll notbepresentedhere.) ►DNAdetectionwasperformedbyusingunmodifiedandAPTES-modifiedsurfaceaswellandvisualizedwithYOYO-1fluorescenceupon200nMdoublestrandedDNAbinding. ►SurfaceadsorptiontendencyofYOYO-1wasofferedasatestingmethodofpropersurfacecoverage,forsensorswithglass-basedactivesurfaces.

  5. Y.ADIGUZEL, 12.08.2014 Materials & Methods ►Fluorescentmicroscopeimageswerecapturedat200ISO,withtheGFPfilter.Brightnessoftheimageswasincreased64%,forclarity. ►Invalidregionswereremovedfromtheoriginalimages. ►YOYO-1concentrationinthecontrolswere3foldsmore,asaresultofexperimentalprocedure.Brightnessoftherelevantimageswerereduced66%,toequilibrateYOYO-1intensities. ►RGBprofileswereplottedonadescendingdiagonallinethatwasdrawnontheimages,withtheplugintoolsoftheImageJ1.47v. Y.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  6. Y.ADIGUZEL, 12.08.2014 Results • APTESModificationof theSurface  • Scanningelectronmicroscopic(SEM)characterizationbyQUANTA 400FField EmissionSEM oftheMiddleEastTechnicalUniversity, CentralLab: 40 µm 40 µm Unmodifiedsurface APTES-modified surface 2 0.5µm Unmodifiedsurface APTES-modified SEMimages ofunmodifiedandAPsTuErfaSc-modifiedglassmicrofiber papersurfaces. Sampleswerecoatedwithgold-palladium.

  7. Y.ADIGUZEL, 12.08.2014 Results * *“Differenttreatment”standsfor:PresenceorabsenceofAPTES- modification;orapplicationofYOYO-1preliminarytoDNA;or changingincubationdurationsafterwashing,followingtreatments. BackgroundImage:Exemplaryimage forYOYO-1interactionwithDNAonglassmicrofiberfilterpaper.

  8. Y.ADIGUZEL, 12.08.2014 (Figure1) RGBprofileplotsof fluorescentmicroscopeimages of APTES-modifiedsamplesthatweretreatedwith YOYO-1only,orYOYO-1and DNA,inthegiven order,followedbywashingand incubation. RGB profileplotsofthedata,publishedinY.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  9. Y.ADIGUZEL, 12.08.2014 Interpretation ofFigure1 ►YOYO-1leadstoacharacteristicgreenfluorescence,whenintercalatedintothedouble-strandedDNA,ascanbeseenthroughthegreenchannelvaluesin(a)comparedtotherest. RGB profileplotsofthedata,publishedinY.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  10. Y.ADIGUZEL, 12.08.2014 (Figure2) RGBprofileplotsof fluorescentmicroscopeimages of unmodifiedsamplesthatweretreatedwithYOYO-1 only,orYOYO-1andDNA,inthegivenorder,followed bywashingand incubation. RGB profileplotsofthedata,publishedinY.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  11. Y.ADIGUZEL, 12.08.2014 Interpretation ofFigure2 ►GreenYOYO-1fluorescencewasaccompaniedandevendominatedbyredfluorescence,whensurfacewasunmodified.IntensityofthesefluorescencesignalsrosewithDNAaddition. RGB profileplotsofthedata,publishedinY.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  12. Y.ADIGUZEL, 12.08.2014 RGBprofileplotsof fluorescentmicroscopeimages of YOYO-1onAPTES-modifiedversusunmodifiedsamples. (Figure3) InterpretationofFigure3 ►GreenYOYO-1fluorescencewasaccompaniedbyredfluorescence,whensurfacewasunmodified,duetoadsorptionofYOYO-1directlyontotheglasssurface. ►Aptcoverageoftheglasssurfacesisimportantforsurfacemodificationsandfollowinginteractionkinetics. ►So,YOYO-1canbeusedtotestpropersurfacecoverage. RGB profileplotsofthedata,publishedinY.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  13. Y.ADIGUZEL, 12.08.2014 Conclusionsof the PreviousWork ►YOYO-1leadstogreenfluorescence,whenintercalatedintodouble-strandedDNA,onAPTES-modifiedglasssurface. ►Sensitivitywasfoundtobe2nM(Datawasnotpresentedhere.) ►YOYO-1sourcedemissionremainedwithlittleintensityloss,aftersamplewashingandincubationfor2.5hmore.Thislosswasremediatedtill15h. ►YOYO-1fluorescencewasaccompaniedanddominatedbyredfluoresĐeŶĐe, wheŶ surfaĐe wasuŶŵodified. It’sproďaďle thatthiswassourcedbyYOYO-1adsorptiononsurface. ►YOYO-1sourcedemissiondidnotundergoanyintensity loss,aftersamplewashingandincubationfor2.5hmore. ►Aptcoverageoftheglasssurfacesisimportantforsurfacemodificationsandfollowinginteractionkinetics. ►YOYO-1canbeusedtotestpropersurfacecoverage. Y.AdiguzelandH.Kulah,(2014)Biosens.Bioel. 54:27.

  14. Y.ADIGUZEL, 12.08.2014 FurtherAnalysis: Analysisof R/G Mean Ratios

  15. Y.ADIGUZEL, 12.08.2014 FurtherAnalysis: • Analysis of R/G Mean Ratios • Materials& Methods • ►Fluorescentmicroscopeimageswerecapturedat1600ISOand200ISO,withtheGFPfilter. • ►Invalidregionswereremovedfromtheoriginalimages. • ►YOYO-1concentrationinthecontrolswere3foldsmore,dueexperimentalprocedure.Brightnessofrelevantimageswerereduced66%,toequilibrateYOYO-1intensities. • ►RGBprofileswereplottedonadescendingdiagonallinethatwasdrawnontheimages,withtheplugintoolsoftheImageJ1.47v. • ►R/GmeanratioswerecalculatedfromtheRGBmeanvaluesofthecolorhistogramresults,whichwasobtainedbyusingthecolorhistogrampluginofImageJ1.47v.

  16. Y.ADIGUZEL, 12.08.2014 Changesin R/G Mean Ratios by Exposure

  17. Y.ADIGUZEL, 12.08.2014 Discussion for FurtherAnalysis ►Duringimaging,changingtheISOfrom1600to200,decreasestheexposure8folds.ThischangeisnormallynotexpectedtoinfluenceR/GmeanratiosoftheRGBvalues. ►WhenimagingYOYO-1fluorescenceontheAPTES-modifiedsamples,decreasingtheexposurelevel8foldsincreasedtheR/Gmeanratios,forthemeasurementsincludinganduntiltheoneat4hofincubation. ►Conversely,decreasingtheexposurelevel8foldsleadtodiminishedR/Gmeanratios,incaseof12hincubation. ►ThislatterobservationcouldhaveimplicationsinYOYO-1interactionwiththesurfacesandDNA,suchasdifferencesinthefluorescentemissiongainsofmolecules,orvaryingemissiongainsofthesamemoleculeatdiversewavelengths.

  18. Y.ADIGUZEL, 12.08.2014 Paper-BasedMicrofluidics • PreviousStudies • ►Oyamaetal.[(2012)LabChip12:5155)]previouslyreportedelectroosmoticlateralflowimmunoassay,withglassfibersheets. • ►Inrelationtothiswork,theyconfirmedthelinearrelationshipbetweentheconcentrationofanalyteandtheresultingfluorescenceintensityfromtheimmunoassayofC-reactiveproteinandinsulin. • ►Fangetal.[(2014)LabChip14:911)]reportedpaper-basedmicrofluidicswithhighresolutioncutonaglassfibermembraneforbioassays. • ►Microchannelswereproducedwithacommoncutter. • ►Bythismeans,theycreatedastarmicro-arrayformat • ofmultiplexedurinetests.

  19. Y.ADIGUZEL, 12.08.2014 Paper-BasedMicrofluidics • CurrentApproach • ►Itispossibletorealizeglassmicrofiberfilterpaper-basedmicrofluidicsapproaches,utilizingbothsidesofthefilterpapertobuiltinterconnectedfluidchannels. • ►Accordingly,water-baseddye,appliedformamacrochannelonthefrontsideoftheglassmicrofiberpaper,istransmittedtothebackside,onlyatthecross-sectionofanotherchannel,diagonaltotheoneonthefront: • Channelatthebackside • Channelonthefrontside • 2.5cm • ►MEMS-basedcoatingislikelytoovercomethislimitation.

  20. Y.ADIGUZEL, 12.08.2014 Summary ► Progresson the Studies onVisualDetection and Surface ModificationTestingof Glass MicrofiberFilterBased Biosensoris presentedthrough: 1-)Data ofthepreviousworkfor DNAdetectionbyYOYO-1 fluorescenceon glassmicrofiberpaper[Y.AdiguzelandH. Kulah,(2014) Biosens.Bioel. 54:27],which was presented& discussedthroughthere-drawnRGBprofileplotsof thedata. 2-)Performingfurtheranalysisof thedata,by calculatingthe R/G meanratios. R/G mean ratioswerechanging,alongwith theISOlevelthatresultedin 8 folds lessexposure. This outcomecan haverelevancetosomephysicalphenomena, and YOYO-1interactionkineticswith DNA. 3-)Initialand earlypaper-based microfluidicsapproachesthat areutilizingglass microfiberpapers were presented.

  21. Y.ADIGUZEL, 12.08.2014 Acknowledgments We would liketothanktothe: ►EuropeanUnion 7th Framework Program,Capacities SpecialProgram- "ResearchPotentials"area (REGPOT), by theframeworkof call:FP7-REGPOT- 2009-1"METU-MEMSResearchandApplications Center(METU-MEMS)“, for financialsupport. ►IstanbulKemerburgazUniversity,for financial support. ►BioMEMS group of METU-MEMS.

  22. Let Us Meet Again We welcome you all to our future conferences of OMICS Group International Please Visit:www.omicsgroup.com www.conferenceseries.com www.pharmaceuticalconferences.com

More Related