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Mutated MSRBP Activity Determination by HPLC Procedure

The activity of mutated cysteine 181 serine MSRBP was analyzed using an HPLC procedure with a total of 6.8 µM C181S MSRBP1 in the reaction system. Refer to 'Materials and Methods' for detailed procedure.

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Mutated MSRBP Activity Determination by HPLC Procedure

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  1. Figure S1 Dabsyl- Met-R-SO Dabsyl- Met-S-SO + MSRB (C181S) + DTT Fig. S1 The activity of mutated MSRB (C181S) was determined by HPLC procedure. Total 6.8 µM C181S MSRB1 was added to the standard reaction system. The detailed HPLC procedure was referred to ‘Materials and methods’

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