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Control of Microbial Growth

Control of Microbial Growth. Chemical and Chemotherapeutic Agents. Chemical Control. MH (one per group) Culture Pick one of 2 Coordinate with other table Quadrants Label for solutions (A, B, C, D) Label bacteria used Swab Confluent growth streak Rotate 90 ° Filter Disk

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Control of Microbial Growth

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  1. Control of Microbial Growth Chemical and Chemotherapeutic Agents

  2. Chemical Control • MH (one per group) • Culture • Pick one of 2 • Coordinate with other table • Quadrants • Label for solutions • (A, B, C, D) • Label bacteria used • Swab • Confluent growth streak • Rotate 90° • Filter Disk • Dip in proper solution • Place in appropriate quad • Repeat for all 4 quads A C D B

  3. Chemotherapeutic Agents • MH agar plate • Group: Pairs • Swab • Confluent • 2x (90°) • AB discs • dispenser • seat discs

  4. Isopropranol H2O2 Clorox Lab cleaner Other Lysol Betadine Chlorhexidine Denature, dissolve PM Oxidative decarboxylation Oxidation Denature, dissolve PM Others Denature, dissolve PM Oxidation Disrupts plasma membranes Chemical Agents MOA

  5. Minimum Inhibitory Concentration MIC

  6. KB: measurements

  7. Results • Zones of inhibition • Measure diameter • Units: mm • Results • Table of MIC • Interpretation • Susceptible • Intermediate • Resistant

  8. Naldixic acid TMPS Gentamycin Vancomycin Penicillin Sulfisoxazole Tetracycline Azithromycin Inhibit DNA gyrase Inhibit Folic acid synthesis 30S binding/interferes with mRNA Inhibit cell wall synthesis Inhibit cell wall synthesis PABA analog, blocks folic acid 30S binding/interferes with tRNA 50S binding/ prevents protein elongation Chemotherapeutic Agents MOA

  9. Additive and Synergistic Effects of AB • Combination Therapy • Reduce resistance • Lower dosage • Enhance activity • Results • Zones of inhibition • Effects • Additive • Synergistic

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