Quantifying Odor Tuning Curves in Water-Deprived Mice: Insights from ORN Responses
This study investigates the tuning curve shapes of olfactory receptors in water-deprived mice through a two-choice test involving familiar odor cues (Methyl Butyrate and Methyl Valerate). Using a water delivery system, we trained the mice to associate specific odors with rewards. Different concentrations of odors were tested to quantify performance differences between wild-type and heterozygous mice. Results indicate that wild-type mice demonstrate superior performance at certain concentrations, revealing crucial insights into olfactory processing and the impact of disrupted projections on odor perception.
Quantifying Odor Tuning Curves in Water-Deprived Mice: Insights from ORN Responses
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Presentation Transcript
Wild type Kir Mice ORNs G MC ORNs G MC
Quantify the tuning curve shape by computing S (Sparseness) Index. (Michelle L Schlief & Rachel Wilson, 2007)
Interval Water Delivery Water Port IR Cross Two-choice Test • Initial Training • Motivation to water- Training water-deprived (about 1.5 ml per day) mice. • Mice familiar with the boxes. • Interest to holes- Mice were rewarded with water for every nose poke into the sniff port. • Training 1- Associate the odor with the water port. • a. Odor A with water A. • b. Odor B with water B. • 5. Two choice WaterA Odor WaterB 3.
Methyl Butyrate Methyl Valerate E-2 E-3 E-4 E-5 E-6
E-1 E-2 E-3 E-4 E-5 E-6 E-7 E-8 E-9 E-2 Wt (normal projection) mice show better performance than Het (disrupted projection) at some concentration.
Cross-habitation Odor presenting sequence: MO MO MO MO MO OdorA OdorA OdorA OdorA OdorA OdorB OdorB Waiting time = 5 min, Odor presenting duration = 2 min, Interval = 3 mins Flow rate: total: 300ml/min each box, 30ml/min for odor deliver each box.
Amyl Acetate Vs Heptanal B6, 12mice
Methyl Butyrate vs Methyl Valerate B6, 12mice
Amyl Acetate Vs Heptanal WT/HET 9 animals
Amyl Acetate vs Heptanal HET/HET 9 animals
Methyl Butyrate vs Methyl Valerate WT/HET 9 animals
Methyl Butyrate vs Methyl Valerate HET/HET 9 animals
Mixture Dilution Amyl Acetate vs. Heptanal
