XCC3227

1. Supplemental Figure 1: Determination of transcription start sites of a subset of σE target promoters by primer extension assays. Total RNA from WT, DrpoE, DrseA, DmucD and WT or DrpoE strains containing control plasmid p917 (-) or σE-overexpressing plasmid p917-rpoE (+) were used as templates in primer extension experiments when suited. Total RNAs were obtained from cells incubated at 30°C or after a 60 min. shift at 35°C. Primers K, xcc1535-EXT, xcc0964-EXT, hrpF-EXT, hpa1-EXT, xcc1246, ompW-EXT, pqqA-EXT, xcc0401-EXT, prc-EXT, xcc4186-EXT and xcc3227-EXT were 5' end labeled with 32P and extended with reverse transcriptase to map their corresponding gene promoter sequences. The arrowheads indicate the bands corresponding to the main start sites observed.

2. A C G T A C G T A C G T A A A A C C C C G G G G T T T T A C G T A A A A C C C C G G G G T T T T DrseA wt Fig. S1 DrpoE (-) DrpoE (+) DrpoE DrpoE DrseA wt (+) wt (-) DrpoE DrpoE DrseA wt (+) wt (-) wt wt wt wt DrpoE DrpoE DrseA PrseA wt (+) wt (-) wt wt 30°C 35°C 30°C 30°C 30°C 35°C 30°C 35°C XCC1535 prc XCC3227 mucD DrpoE DrpoE DrseA wt (+) wt (-) DrpoE DrseA wt (+) wt (-) wt wt wt 30°C 35°C 30°C 30°C 30°C DrpoE DrseA wt (+) wt (-) wt 30°C XCC1246 ompW hpaI DrpoE (-) DrpoE DrpoE (+) DrseA wt (+) wt (-) DmucD DrpoE DrseA DrpoE wt (+) DrseA wt wt (-) wt (+) wt (-) wt DrpoE DrpoE DrseA wt (+) DrseA wt (+) wt (-) wt (-) 30°C 30°C wt wt 30°C 30°C 30°C 30°C 30°C 30°C 30°C 30°C XCC0964 XCC0401 XCC4186 hrpF pqqA