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Electron Transport and Oxidative Phosphorylation: The Rules of Coupling!

This question set covers the important concepts of electron transport and oxidative phosphorylation in cellular respiration. It discusses the rules of coupling, electron transport chain, F1F0ATPase, uncouplers, oxygen block, alternative electron acceptor, blocking F0, and the effects of cyanide.

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Electron Transport and Oxidative Phosphorylation: The Rules of Coupling!

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  1. Questions 6 - Electron Transport and Oxidative Phosphorylation

  2. 6. The Rules of Coupling! • Which of the following statements is INCORRECT? • Electrons can move down the electron transport chain even if proton pumping from the matrix can not occur. • Protons are only pumped from the matrix if electrons are passed down the electron transport chain. • ATP synthesis at the F1ATPase requires both ADP and phosphate • Protons will only come in through the F0F1ATPase if ATP is simultaneously being made from ADP • Protons can pass freely across the outer mitochondrial membrane coupling says no coupling says yes fundamental yes important rule no passing INNER… BUT OUTER!

  3. 7. Electron Transport Chain • Which of the following statements concerning electron transport and proton pumping is INCORRECT? • Proton release occurs when electron carriers receive electrons from hydrogen carriers • Cytochrome c carries only electrons • Ubiquinone carries hydrogens from Complex I to Complex III • Oxygen is consumed on the matrix side of the inner mitochondrial membrane • The actual protons that move out of the matrix during electron transport come exclusively from the hydrogens on NADH yes as H gives e’s, protons are let loose it just does! I to III, or II to III yes if 10 get pumped out for every NADH, can’t ALL come from NADH

  4. 8. The F1F0ATPase • Which description of the components in the F0F1ATPase is INCORRECT? • The gamma subunit rotates as protons enter the matrix • Portions of the F0 channel rotate as protons pass through it • The stator portion prevents free rotation of the alpha-and beta-subunits • ATP is made on an active site on the gamma subunit • The F1ATPase portion is located inside the matrix long spindly thing that rotates Cylinders do rotate Yes, that’s what stator means ATP is made on betas yes

  5. 9. Uncoupler dissipation of the gradient → no drive for ATP synthesis but no brakes on proton pumping → fuel oxidation increases An uncoupler

  6. 10. No oxygen block electron transport chain → no proton pumping → proton gradient gets used up and not regenerated → ATP synthesis grinds to halt A lack of oxygen

  7. 11. Alternative Electron Acceptor • Exposure to a compound which accepts electrons directly from cytochrome c electron transport keeps going (but not through Complex IV) → no oxygen consumption → still get a proton gradient → still get ATP

  8. 12. Blocking F0 An inhibitor of proton movement through the F0 channel wont dissipate the proton gradient → wont be able to make ATP → back pressure stops electron transport and fuel oxidation

  9. 13. Cyanide An inhibitor of electron transport at Complex IV block electron transport chain → no proton pumping → proton gradient gets used up and not regenerated → ATP synthesis grinds to halt

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