High Throughput Screening To Identify Effectors Of The

1. High Throughput Screening To Identify Effectors Of The Bone Morphogenetic Protein Signalling Pathway. Matthew Benson*, Karen Soegaard*, Angela Lee*, Alison Howarth**, DilairBanan***,Daniel Ebner**, and Shoumo Bhattacharya*. *Radcliffe Department of Medicine, Division of Cardiovascular Medicine, University of Oxford ** Target Discovery Institute, University of Oxford ** *High throughput Genomics Facility, WTCHG, University of Oxford Background The transforming growth factor β (TGFβ) superfamily of proteins, which includes the Bone morphogenetic protein (BMP) subgroup, encompasses a functionally diverse group of glycosylated, extracellular matrix-associated signalling molecules [1]. Fine control of the BMP-signalling process via an intricate mechanism of antagonists, receptor functional regulation and BMP-target gene methylation is fundamental to a wide variety of cellular processes. Disruption of this control underlies numerous developmental disorders and human diseases including abnormal cardiac development, fibrosis and pulmonary hypertension [2]. Furthermore, maintenance of the fine balance between TGFβ (increases cardiac fibrosis) and BMP (decreases cardiac fibrosis) is highly relevant to myocardial homeostasis [3]. We have developed a high-throughput cell-based assay to identify genes which affect the BMP-signalling pathway. High-throughput screening protocols To identify novel effectors of, and putative novel drug targets for BMP signalling, using the BRE-luc C2C12 cell line we performed large scale RNAi cell-based assays (9968 genes) SiRNA inhibitors were chosen from members of the druggable genome or potential drug targets (primarily kinases and GPCRs). Day Three Day One Day Two Day Four 2500 C2C12 Bre-luc cells in 50ul media (10% FCS)/well 0.4ul RNAimax in 20ul optimem siRNA library in 384 well plates 1x384 well, white plate Media removed, cells washed in PBS, steadylite added and luciferase signal read 1x384 well plate Media removed and replaced with 0.1% FBS media containing BMP4 (3ng/ml) 20 min incubation then 18ul transferred to new plate Repeated in duplicate at 25nM siRNA final conc. control siRNAs The screening data was analysed using CellHTS2 (web-cellhts2.dkfz.de). 653 siRNAswere classified as hits having a B-Score of +/- 3 Therapeutic targets in the BMP signalling pathway Development of a TGFβ luciferase reporter C2C12 cell line to assess off-target effects To confirm that the effects seen on the BRE-luc reporter where A) specific for BMP-signalling and B) not targeting luciferase directly the TGFβ responsive element from the PAI-1 promoter, CAGA12, was cloned into the same plasmid backbone as the BRE-luc reporter. This plasmid was then transfected into C2C12 cells together with a plasmid expressing G418 resistance and a stable cell line was produced by multiple rounds of selection using 800ug/ml G418. BMP signalling proceeds initially by propeptide cleavage via Furin/PCSK5. BMP antagonists such as chordin, gremlin, noggin are also activated by propeptide cleavage (TLL-1). BMP binds to receptors on the cell surface causing dimerisation Activated BMP receptors phosphorylate SMAD proteins which regulate the potentiation of BMP-signalling via the R-SMADS. Phospho-SMADS bind to SMAD4 and enter the nucleus The SMAD-complex enters the nucleus and induces gene expression. Structure of the CAGA12-luc reporter TGFβ response (uM) Adapted from Walsh et al., Trends in Cell Biology, 2010 BMP4 response (ng/ml) Having established an assay to assess non-BMP driven off-target effects, 580 siRNA hits were retested against BRE-luc and CAGA12-luciferase in C2C12 cells. • Of the 580 hits, RNA inhibition of 102 genes caused an increase in BMP-signalling whilst decreasing or not affecting TGFβ signalling BMP response of the C2C12 BRE-luc cell line We utilised a BMP-responsive luciferase reporter C2C12 cell line called BRE-luc [5, 6] to identify molecules and siRNA which affected BMP signalling. BRE contains two copies of the Id1-promoter (−1105/1080) fragment fused to two copies of the Id1- (−1052/−1032) fragment cloned into pGL3 (Promega). By using a BMP4 concentration which gave 75% of the maximal luciferase output (4ng/ml) we can identify both inhibitors and activators in one screen. Identification of genes specifically regulated by BMP4 and TGFβ1 in C2C12 cells BMP4 dose response of the BRE-luc C2C12 cell line. 5000 cells were grown per well in 96 well plates in DMEM, 10%FCS, 2mM L-Glut, Pen/Strep and 700ug/ml G418. After 24h the media was changed to low serum media (identical to previous with only 0.1% FCS) containing various doses of BMP4. After 24h the media was removed, the cells were washed with PBS and 50uL of Steadylite (PE) added. After a 20min incubation luminescence was read using a BMG Polarstar plate reader. In order to identify genes that are specifically regulated by BMP4 and TGFβ1 we performed a microarray, comparing gene expression in C2C12 cells stimulated for 2 or 18 h, with or without BMP4 or TGFβ1. Illumina WG6 MouseWG-6 v2 Expression BeadChips were used fro the array and differentially expressed genes were identified as showing more than 2 fold change in expression levels at p<0.05, compared to unstimulated control. A total of 544 unique genes were identified as differentially regulated after BMP4 and TGFβ1 stimulation of C2C12 cells, as specified in the table. We have selected 3-4 of the most regulated genes from each stimulus and each time point (13 in total). These we will test in QPCR assays, with the goal of using the QPCR assays to check that the knockdown, with the siRNAs identified in the screen, specifically affects the BMP signalling pathway and not the TGFβ signalling pathway. Structure of the BRE-lucreporter. SBE, Smad-binding element Inhibition of the BRE-luc reporter activity by using SMAD4 siRNA inhibition The table shows the number of genes differentially regulated by the two stimuli, combining the two time points. Interestingly, 10 of the genes differentially regulated by both TGFβ1 and BMP4 were regulated in opposite directions by the two stimuli. Having established the conditions required for screening we optimised the control conditions required for performing the screen. The BMP-signalling pathway was inhibited either through treatment by siRNA knockdown of SMAD4. siRNA inhibition Conclusion We have developed a cell-based high throughput assay capable of identifying specific gene modulators of BMP signalling. After screening clinically relevant siRNAs (9968 genes), we have identified several promising targets for future investigation that could provide novel insights into the role of BMP in fibrosis and pulmonary artery hypertension. Dharmafect 3 RNAiMax kDa A B C D A B C D A B C D E Utr A B C D A B C D A B C D E Utr Acknowledgments (Arial, 36 points, bold) In this template, acknowledgments are set in Arial, 32 points. Try to keep the acknowledgments to one or two lines. Z’=0.62 130 95 72 Blot SMAD4 55 36 28 17 siRNAnM 25 12.5 5 25 12.5 5 130 relative light units 95 72 Blot β-tubulin 55 36 28 17 • Protein levels of SMAD4 are reduced in response to siRNA treatment. • 5000 cells grown in DMEM, 10%FCS, 2mM L-Glutamine were reverse transfected using 25, 12.5 or 5 nM SMAD4 siRNA and 0.05ul/well (A), 0.075ul/well (B), 0.1ul/well (C) or 0.2ul/well (D) of Dharmafect 3 (Thermo) or RNAimax (Life Technologies). • (E) represents non target control siRNA (25nM) with 0.2ul/well transfection reagent. (Utr), untransfected cells • After 24h the media was changed to low serum media containing 4ng/ml BMP4. After 48h the media was removed, the cells were washed with PBS and lysed in 1xLaemmli buffer. 60ug of protein/lane was western blotted for SMAD4. The blots were stripped and probed for β-tubulin • BRE-lucluminescense is reduced in response to 25nM SMAD4 siRNA (Dharmacon). • 2500 cells grown in DMEM, 10%FCS, 2mM L-Glutamine were reverse transfected using 25nM siRNA and 0.1ul/well RNAimax (Life Technologies) and placed into 384 well plates. After 24h the media was changed to low serum media containing 4ng/ml BMP4. After 48h the media was removed, the cells were washed with PBS and 25ul of Steadylite (PE) added. After a 20min incubation luminescence was read using a BMG Polarstar plate reader. NTC, non target control siRNA (Dharmacon) • References • 1) Wu, M.Y. and C.S. Hill, Tgf-beta superfamily signaling in embryonic development and homeostasis.Dev Cell, 2009. 16(3): p. 329-43. • Walsh, D.W., et al., Extracellular BMP-antagonist regulation in development and disease: tied up in knots. Trends Cell Biol, 2010. 20(5): p. 244-56. • Zeisberg, E.M., et al., Endothelial-to-mesenchymal transition contributes to cardiac fibrosis. Nat Med, 2007. 13(8): p. 952-61. • 4) Sieber, C., et al., Recent advances in BMP receptor signaling. Cytokine Growth Factor Rev, 2009. 20(5-6): p. 343-55. • Korchynskyi, O. and P. ten Dijke, Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter. J BiolChem, 2002. 277(7): p. 4883-91. • Herrera, B. and G.J. Inman, A rapid and sensitive bioassay for the simultaneous measuremntof multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol, 2009. 10: p. 20.