1 / 12

Comparative Analysis of Serum Protein Profiles in Healthy and P.falciparum Diseased Individuals

This study presents a comprehensive analysis of 2D gel images from serum protein samples of healthy controls and individuals infected with P.falciparum. Key steps include project setup, spot detection and quantification, and expression profile analysis. The results show significant differential expression patterns, with identified spots reflecting up-regulation and down-regulation in the diseased group compared to healthy controls. Statistical analyses, including hierarchical clustering and scatter plots, provide insights into protein correlations and expression trends relevant to malaria research.

rufus
Télécharger la présentation

Comparative Analysis of Serum Protein Profiles in Healthy and P.falciparum Diseased Individuals

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Set of gel images for analysis Healthy Control Case 2D gel images of Serum protein sample

  2. Steps followed in gel analysis 1) Set up Project 2) Detect and Quantify Spots 3) Analyze Expression Profiles 4) Present Results

  3. ANALYSIS, RESULT AND DATA INTERPRETATION

  4. 1. Project Summary: Groups Control(1 image) Control - Healthy person 349spots transferred from fused image 97 spots cancelled Diseased (1 image) P.falciparum diseased serum sample 349 spots transferred from fused image 97 spots cancelled Vector Matching : 109

  5. 1. Project Summary: Profile Spot Information Control Diseased Spot ID : 1999 pI : 5.26 MW : 65.1kDal 3D view 3D view Control P.fal Fused Spot ID : 2082 pI : 5.28 MW : 44.8kDal 3D view 3D view Control P.fal Fused

  6. 3.Figure showing Molecular weight to pI pI index Molecular weight (kDal) Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal Spot ID: 1999 pI : 5.26 Mol. weight : 65.2 kDal

  7. 4.1. Differential expression : Up regulation Up regulation x Folds or above No. of spots showing up regulation

  8. 4.2. expression profile: Up regulation(8-10 folds up) -11 spots Mean normalized value of group 2 (P.falciparum diseased person) Ratio = Mean normalized value of group-Control(Healthy person)

  9. 4.3.Differential expression : Down regulation Down regulation x Folds or above No. of spots showing up regulation

  10. 4.4.Expression profile: Down regulation(9-10 folds down) - 9 spots Mean normalized value of group 2 (P.falciparum diseased person) Ratio = Mean normalized value of group-Control(Healthy person)

  11. 5.Cluster Analysis Hierarchical Cluster Analysis HCL Tree It helps in finding correlated protein It provides information about similarly expressed protein.

  12. 6.Scatter plot

More Related