1 / 4

409_C_1 (or -6C) ‏

409_C_1 (or -6C) ‏. 409_A. 409_C. 409_G (WT) ‏. 409_T. a: -801.51 c: -863.91 g: -857.45 t: -859.32. 407_G_1 (or -8G) ‏. 407_C. 407_G. 407_T. 407_A (WT) ‏. g: -876.05 a: -870.01 c: -870.33 t: -869.29. 422_C_1 (or +8C) ‏. 422_C. 422_G. 422_T. 422_A (WT) ‏.

sacha-vazquez
Télécharger la présentation

409_C_1 (or -6C) ‏

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 409_C_1 (or -6C)‏ 409_A 409_C 409_G (WT)‏ 409_T a: -801.51 c: -863.91 g: -857.45 t: -859.32

  2. 407_G_1 (or -8G)‏ 407_C 407_G 407_T 407_A (WT)‏ g: -876.05 a: -870.01 c: -870.33 t: -869.29

  3. 422_C_1 (or +8C)‏ 422_C 422_G 422_T 422_A (WT)‏ c: -849.70 g: -838.41 a: -835.20 t: -838.70

  4. (-) TGAGGAGGTT TCTCTGTAAG (+)‏ Design accessed intended position, wt didn’t originally cut it Designs that make protein nonspecific at position WT doesn’t really care -10, -5, +5, +8, +9, +10 -9C, -8T, -8G, -6C, -3 (T), +3 (A)‏ Most important remaining Some off-target activity from designs Design made more specific +5C, +8C, +8A -9T, -9G, -7, -6A, -4C, -4T, +4, +6, +7C, +7G -6T, -4A, +7A

More Related