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Microarray Technique, Analysis, and Applications in Dermatology

Microarray Technique, Analysis, and Applications in Dermatology. Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University of Pittsburgh Medical Center; 2 Virginia Commonwealth University. Microarray: Introduction. Used for

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Microarray Technique, Analysis, and Applications in Dermatology

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  1. Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park1 and Alex G Ortega-Loayza2 1Department of Dermatology, University of Pittsburgh Medical Center; 2Virginia Commonwealth University

  2. Microarray: Introduction • Used for • Assessing gene expression levels • Genome-wide studies and genotyping • Evaluating microRNA levels

  3. How do microarrays work? • Microarray chips are created that contain many thousands of small DNA segments (probes) • Probes are arranged in a known and orderly fashion (the “array”) • Sample mRNA or its corresponding cDNA is labeled and exposed to the DNA probe on the chip • If a given gene is expressed, the mRNA hybridizes to the DNA segment on the chip and can be detected

  4. Microarrays • Types of array platforms: • Filter arrays (i.e., “macroarrays”) • Lower probe density compared to spotted or oligonucleotide arrays • Lower sensitivity (e.g., low-abundance sequences may be missed) • Spotted glass slide arrays • In situ synthesized oligonucleotide arrays • Common probes • Complementary DNA (cDNA) • Oligonucleotides

  5. Oligonucleotide arrays Control cells Experimental cells Hybridize targets to probes Cy3 Cy5 Targets

  6. cDNA arrays Control cells Experimental cells Hybridize targets to probes Cy3 Cy5 Targets

  7. Applications in dermatology • Melanoma • Cutaneous T-cell lymphoma • Psoriasis • Scleroderma • Lupus erythematosus • Others

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