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Competition Analysis of PhoP Binding to glnA, glnII, and amtB Promoters by EMSA

This analysis utilizes Electrophoretic Mobility Shift Assay (EMSA) to investigate the competition for PhoP binding at the glnA, glnII, and amtB promoter regions. It presents data comparing the binding of the PhoP protein (GST-PhoPDBD at 0.5 µM) with and without the presence of excess unlabeled probe. The results highlight the impact of competition on the formation of shifted bands, indicative of protein-DNA interactions. This research contributes to understanding how PhoP regulates gene expression linked to nitrogen metabolism.

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Competition Analysis of PhoP Binding to glnA, glnII, and amtB Promoters by EMSA

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  1. PhoP P C PhoP PhoP P C P C glnII glnA amtB Figure S1. Analysis by EMSA of competition by excess unlabelled probe of binding of PhoP to the glnA, glnII and amtB promoters. P: probe without protein; PhoP: probe with GST-PhoPDBD protein (0.5 µM ); C: Control with GST-PhoPDBD protein (0.5 µM) and an excess of unlabeled probe. Note the competition with the labeled probe in the formation of the shifted band.

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