100+ 2d lc ms ms PowerPoint (PPT) Presentations, 2d lc ms ms PPTs

2d lc ms ms - PowerPoint PPT Presentation

2D-Gel Analysis

2D-Gel Analysis. Jennifer Wagner. Image retrieved from http://en.wikipedia.org/wiki/File:Coomassie-2D-Gels.jpg. 2D-gel analysis. Goals: 1)To characterize and quantify all the proteins in a particular sample

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377 views • 36 slides

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation. Column Technology Inc.,. Part I. Comparison of salt and pH step gradient. pH Based 2D-LC-MS/MS: pH 3.0-8.0, Buffer (5-10mM) v.s. Salt Based 2D-LC-MS/MS: salt 0-2000 mM, pH ~2.5.

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271 views • 13 slides

Phosphopeptides identification

Phosphopeptides identification. Column technology Inc., WWW.columntechnology .com. SAX for phosphopeptide separation. Phosphopeptides have lower pIs then non-phosphopeptides. Off line pH gradient follow by reverse phase separation. Peptides fractionated by pH step gradient

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151 views • 8 slides

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2d lc ms ms - PowerPoint PPT Presentation

2D-Gel Analysis

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Phosphopeptides identification