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In Vitro Differentiation of T Cells

In vitro differentiation of T cells from naïve CD4 T cells is achieved by stimulating their T cell receptor (TCR) in the presence of appropriate cytokines. Here we describe a comprehensive protocol for in vitro differentiation of Th0, Th1, Th2, Th17 and iTreg T cells from mouse CD4 T cells.

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In Vitro Differentiation of T Cells