'Arabinose' diaporamas de présentation

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The Effect of Cyclic Peptides on Sigma E Regulation in Escherichia coli

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pGLO Transformation LAB AP LAB 6

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E.coli expression vector pBAD-DEST49. ? ? ?. Description. pBAD-DEST49 is a 6.2kb vector adapted for use with the Gateway Technology. It is designed for regulated expression of N-terminal HP-thioredoxin fusion proteins in E. coli. Construct - P BAD

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1. Transform bacteria with pGlo plasmid 3. Induce expression of GFP with arabinose

Transformation Lab Creating a GMO by transforming E. coli. 1. Transform bacteria with pGlo plasmid 3. Induce expression of GFP with arabinose. 2. Select for ampicillin resistance. http://www.dnalc.org/harlemdnalab/ BacterialTrans.html. The recombinant pGlo plasmid.

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A jellyfish gene is identified that codes for a protein (GFP) that glows under UV light.

Moving Green Fluorescent Protein. A jellyfish gene is identified that codes for a protein (GFP) that glows under UV light. Researchers clone the gene and insert it into bacteria. Plasmid from E. coli. ara C. Green Fluorescent Protein. O. amp resistance. ara B. P. ara A. ara D.

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Preparing an Overnight Culture of E. coli

Preparing an Overnight Culture of E. coli. 2014. Possible Problems. When there is a mixed culture of red and white (nonexpressing) cells, the white cells will grow faster than those that are using their resources producing red fluorescent protein (mFP) = less mFP produced for purification.

175 views • 5 slides

Preparing an Overnight Culture of E. coli

Preparing an Overnight Culture of E. coli. 2014. Possible Problems. When there is a mixed culture of red and white (nonexpressing) cells, the white cells will grow faster than those that are using their resources producing red fluorescent protein (mFP) = less mFP produced for purification.

57 views • 5 slides