ATOMIC ABSORPTION AND ATOMIC FLUORESCENCE SPECTROMETRY Chap 9 Source Modulation Interferences in Atomic Absorption Spectral Chemical Atomic Fluorescence Spectroscopy. AA Source Modulation. Need to eliminate emission from analyte atoms Source beam is chopped. Chopper placed here.
By halilAA and Atomic Fluorescence Spectroscopy Chapter 9. Sample Atomization Atomic Absorption Instrumentation Interference Atomic Absorption Techniques Atomic Fluorescence Sample Atomization For techniques samples need to be atomized Techniques are useful for element identification
By baryAtomic Absorption Spectroscopy AAS. Comparatively easy to use Low maintenance Low consumables Good for measuring one element at a time. Block Diagram. h n + M → M* Sample is vaporized/atomized by: Flame Electrothermal Vaporizer (ETV). AA Sources- HCL.
By jennAtomic Absorption Spectroscopy AAS. Comparatively easy to use Low maintenance Low consumables Good for measuring one element at a time. Block Diagram. h n + M → M* Sample is vaporized/atomized by: Flame Electrothermal Vaporizer (ETV). AA Sources- HCL.
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Atomic Emission Spectroscopy (AE, AES) Atomic Fluorescence Spectroscopy (AF, AFS). Flame Emission Spectroscopy (AE, AES). excitation และ emission ของ Na atom
Fluorescence spectroscopy. Komal Choudhary Lecturer School of Biotechnology DAVV Indore. Fluorescence spectroscopy. Luminescence It is the emission of light from any substance, and occurs from energetically excited states. It is divided into two categories (1) Fluorescence
Fluorescence Spectroscopy. Quantum Yield f = ________k f ______________ k f + k i + k ec + k ic + k pd + k d k f = rate constant for fluorescence k i = “ “ for intersystem crossing k ec = “ “ for external conversion k ic = “ “ for internal conversion
Fluorescence Spectroscopy. Part I. Background. Perrin-Jablonski diagram. S is singlet and T is triplet. The S 0 state is the ground state and the subscript numbers identify individual states. Energy level of MO. n → p * < p → p * < n → s * < s → p * < s → s *. Singlet & Triplet.
Fluorescence Spectroscopy. Part I. Background. Perrin-Jablonski diagram. S is singlet and T is triplet. The S 0 state is the ground state and the subscript numbers identify individual states. Energy level of MO. n → p * < p → p * < n → s * < s → p * < s → s *. Singlet & Triplet.
AA and Atomic Fluorescence Spectroscopy Chapter 9. Sample Atomization Atomic Absorption Instrumentation Interference Atomic Absorption Techniques Atomic Fluorescence Sample Atomization For techniques samples need to be atomized Techniques are useful for element identification
Atomic Spectroscopy: Atomic Emission Spectroscopy Atomic Absorption Spectroscopy Atomic Fluorescence Spectroscopy * Elemental Analysis * Sample is atomized * Absorption, emission or fluorescence of atoms or ions in the gas phase. Atomic Emission Spectroscopy. Ingle and Crouch,
Emission spectroscopy (Mainly fluorescence spectroscopy). Reading : van Holde , Chapter 11 Homework : due Wednesday, April 2 van Holde 11.2. 11.3, 11.4, 11.5, 11.7, 11.8 Grad students: remember to schedule your presentations.
Emission spectroscopy (mainly fluorescence spectroscopy). Reading : van Holde Chapter 11 Presentation : Nicole Levi: “Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor” Ha et al. PNAS 93 , 2664