'Bac clones' diaporamas de présentation

Goals of the Human Genome Project (1990 ~) Map and sequence the 3,000 Mb human genome

Genomics Databases and Bioinformatics Applications Wailap Victor Ng Institute of Biotechnology in Medicine Institute of Bioinformatics Dept Biotechnology and Lab Science in Medicine National Yang Ming University wvng@ym.edu.tw March 22, 2005. Goals of the Human Genome Project (1990 ~)

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Mapping of BAC-end sequences on the Chicken Genome: American Alligator, Painted Turtle and Emu

Mapping of BAC-end sequences on the Chicken Genome: American Alligator, Painted Turtle and Emu. Charles Chapus. Lab meeting 04/07/2007. Introduction BAC Librairies Methodology Blast results Paired Blast results Conclusion. Introduction BAC Librairies Methodology Blast results

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Oklahoma Medicago truncatula Genomic Sequencing: Progress-Process-People

Oklahoma Medicago truncatula Genomic Sequencing: Progress-Process-People. Dr. Bruce A. Roe Advanced Center for Genome Technology Department of Chemistry and Biochemistry University of Oklahoma broe@ou.edu www.genome.ou.edu. Scientific Advisory Board Meeting

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INDIAN INITIATIVE FOR TOMATO GENOME SEQUENCING Tomato Finishing Workshop

INDIAN INITIATIVE FOR TOMATO GENOME SEQUENCING Tomato Finishing Workshop. T. R. Sharma National Research Centre on Plant Biotechnology Indian Agricultural Research Institute New Delhi -110012 trsharma@nrcpb.org. Tomato Genome Sequencing Project. Spain. USA. USA. Italy. France.

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Jahn Lab Dept. of Plant Breeding & Genetics, Cornell University Original Objectives Years 2-4

Jahn Lab Dept. of Plant Breeding & Genetics, Cornell University Original Objectives Years 2-4 Generate a BAC library from Capsicum frutescens , a major source of disease resistance in pepper Fine map the L locus in Capsicum and assess homology to R3/R7 /potato

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Sequencing the Euchromatic Region of Chromosome 5 of Tomato

Sequencing the Euchromatic Region of Chromosome 5 of Tomato. International Tomato Genome Sequencing Project. Country. USA. Korea. China. UK. India. NL. France. Japan. Spain. USA. USA. Italy. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Chromosome. Mb. 24. 26. 26. 19. 12.

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Sequencing the Euchromatic Region of Chromosome 5 of Tomato

Sequencing the Euchromatic Region of Chromosome 5 of Tomato. International Tomato Genome Sequencing Project. Country. USA. Korea. China. UK. India. NL. France. Japan. Spain. USA. USA. Italy. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Chromosome. Mb. 24. 26. 26. 19. 12.

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Mutation Detection Session FRCPath Preparation Course 9/11/09

Outline the methodology behind array CGH, concentrating on the systems in routine diagnostic use. Describe the advantages and disadvantages of the technology and give examples on how you could go about confirming the significance of a change. Mutation Detection Session

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The Human Genome Project

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The Human Genome Project

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Genetic Map to Physical Map

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Genetic Map to Physical Map

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Charting 2D Gels making use of the different Chlamydomonas databases available

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International Tomato Genome Sequencing Project

International Tomato Genome Sequencing Project. Country. USA. Korea. China. UK. India. NL. France. Japan. Spain. USA. USA. Italy. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Chromosome. Mb. 24. 26. 26. 19. 12. 20. 27. 17. 16. 10. 13. 11. T=220. To sequence.

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International Tomato Genome Sequencing Project. Country. USA. Korea. China. UK. India. NL. France. Japan. Spain. USA. USA. Italy. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Chromosome. Mb. 24. 26. 26. 19. 11. 20. 27. 17. 16. 10. 13. 11. T=219. To sequence.

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Sickle-cell anemia

Sickle-cell anemia. Caused by single bp substitution in b -globin gene. Determine b -globin genotype by RFLP analysis. Other methods to diagnose genotypes. Allele-specific Oligonucleotides (ASOs): Make two oligonucleotide probes: (a) Normal sequence (b) Sequence of mutant allele

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Telomere

CC-NBS-LRR (CNL). Transposable element. F-box. Other gene. Truncated gene. Khipu tandem repeat. Unknown physical distance. Putative location of the minor knob. Location of the major knob. K. K. 43. CNL-B12 (r). 44. CNL-B13 (r). 45. CNL-B14* (r). 46. CNL-B15 (r). 47.

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Sequence Assembly of Medicago Truncatula Chromosomes

Selecting New BAC clones to be sequenced. Selecting New BAC clones to be sequenced. Assembly Pipeline. Conclusion. Clone Selection Efficiency. BAC sequences assembled by PhredPhrap.

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Objective 5. Bioinformatics: Public web-based databases of libraries and maps.

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DISCUSSION

Sub-clone BAC insert into BIBAC vector and transform into rice calli via Agrobacterium. Identify yield-related BAC clones and gene for initial focus of study. Design primer for oxalate-oxidase gene. Field evaluation on the transgenic rice. Polymerase Chain Reaction (PCR).

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