Biophysical tools - PowerPoint PPT Presentation

Chromatography

Chromatography. Partition chromatography. redistribution (partition) of the molecules between the stationary and mobile phase. Plate model of chromatography of mixture of compounds partitioning 1:1 (bold) and 1:3 (italics) between the mobile and stationary phase. .

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218 views • 15 slides

Fluorescence

Fluorescence. Energy diagram. Energy diagram. Quantum Yield - Q. Rate of depopulation. Red shift. Fluorimeter. Time resolved experiment . Spectrofluorimeter. Frequency domain (phase/modulation) . Excitation/ emmission spectrum :. Fluorophores. Intrinsic fluorophores. Extrinsic probes.

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444 views • 9 slides

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