'Capsule stain' diaporamas de présentation

objectives

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Microscopy

Microscopy. Most microorganisms are in the micrometer size range. m = 1 meter cm = centimeter = 1/100m = 10 -2 meters mm = millimeter = 10 -3 meters ? m = micrometer = 10 -6 meters nm = nanometer = 10 -9 meters 1 Angstrom = 10 -10 meters pm = picometer = 10 -12 meters .

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Gram Negative Bacilli

Gram Negative Bacilli. Enterobacteriaceae Family : They include large heterogeneous group of gram negative rods whose natural habitat is the intestinal tract of man or animals. General characteristics : 1- They are short rods with rounded edge 4-5 microns in length,

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Bacterial Staining

Bacterial Staining. General Discussion. Stains. All dyes are salts Ionize Cationic Anionic Techniques Single dyes Multiple dyes Use. Chemical Makeup of Stains. Benzene = organic compound Chromophore = color Auxochrome = ionization properties Benzene + Chromophore = Chromogen

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EXPERIMENT #4

EXPERIMENT #4. ACID FAST STAIN ENDOSPORE STAIN CAPSULE STAIN MOTILITY (Wet mount/Hanging drop). Acid Fast Stains. Primary Stain: Kinyoun Carbolfuchsin (w/ high concentration of phenol). - - - - - -. Non Acid Fast: GRAM +. 2. Acid alcohol: Decolorizer. Acid Fast: Mycobacterium.

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Bacterial Structure (Lab 3 )

Bacterial Structure (Lab 3 ). Rana Alqusumi. Cell wall. Cell membrane. Cytoplasm. Nuclear material. Essential Bacterial Structures. Capsule. Flagella. Pili. Fimbriae. Spore. Particular Bacterial Structures. Bacterial Structure (Capsule). Glycocalyx :

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Differential staining

Differential staining. Spore stain Capsule stain. Spore stain.

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Gram stain: Gram positive streptococcus and Gram negative rod

Gram stain: Gram positive streptococcus and Gram negative rod. Gram stain: Klebsiella pneumoniae. Gram stain: Clostridium acetobutylicum (pink cells are old/dying/dead). Capsule stain: K. pneumoniae (Stains: Methylene blue and nigrosin).

1.37k views • 9 slides