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t-cellbiology/teaching

t-cellbiology/teaching

Simon Davis, Ed Evans: ? 21336. www.t-cellbiology.org/teaching. Methods Course, November 4th. 1. Expression cloning In bacteria In mammalian cells Yeast 2-hybrid screens. N.B. Now rarely used!. Methods Course, November 4th. 2. Protein expression (overview) Why express proteins at all?

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774 views • 57 slides
Therapeutic Uses for Blood Products and Enzyme Therapeutics

Therapeutic Uses for Blood Products and Enzyme Therapeutics

Therapeutic Uses for Blood Products and Enzyme Therapeutics. BIT 230 Walsh Chapter 9. Blood and Blood Products. Source of traditional biologics Blood composed of red blood cells white blood cells platelets plasma (contain cellular elements) - therapeutic proteins come from plasma.

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948 views • 36 slides
Protein glycosylation

Protein glycosylation

Protein glycosylation. Adds another layerof structure and specificity to proteins Can enhance the function of a protein Can extend the lifetime of a protein Can help localize a protein within a cell Can act as a specific antigen. Two types of protein glycosylation. N-acetyl group. glucose.

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660 views • 34 slides
The BAR (Bin/amphiphysin/Rvs) domain is the most conserved feature in amphiphysins from yeast to human and is also found

The BAR (Bin/amphiphysin/Rvs) domain is the most conserved feature in amphiphysins from yeast to human and is also found

BAR Domains as Sensors of Membrane Curvature: The Amphiphysin BAR Structure Brian J. Peter, * Helen M. Kent, * Ian G. Mills, Yvonne Vallis, P. Jonathan G. Butler, Philip R. Evans, Harvey T. McMahon.

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583 views • 25 slides
Selected Products of Bioprocessing

Selected Products of Bioprocessing

Selected Products of Bioprocessing. digging a bit deeper. Farmaceuticals. genetic modification of animals production of therapeutic proteins in milk, eggs or blood. Demand?. >100 protein based drugs are in advanced phases of clinical trials production facilities cost $200m-400m

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322 views • 23 slides
BIOT 2922

BIOT 2922

BIOT 2922. Tissue Culture Methods. Objectives. Work aseptically. Maintain mammalian cells in healthy, uncontaminated state for 2-4 weeks. Manipulate mammalian cells. Cell lines. Chinese hamster ovary (CHO) cells Introduced in the 1960s. Used in a cultured monolayer in culture flasks.

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311 views • 11 slides
3D7 UM

3D7 UM

p=0.14. % adhesion. CHO cells. 30. 30. 30. 30. 30. p=0.0006. Events. Events. Events. Events. Events. % adhesion. 0. 0. 0. 0. 0. 10 0. 10 0. 10 0. 10 0. 10 0. 10 1. 10 1. 10 1. 10 1. 10 1. 10 2. 10 2. 10 2. 10 2. 10 2. 10 3. 10 3. 10 3. 10 3. 10 3. FITC.

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126 views • 1 slides

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