Cos sites - PowerPoint PPT Presentation

l PHAGE

Vector for cloning large DNA inserts. l PHAGE. l DNA. La regione N-J (circa 19 kb) puÃ² essere rimossa o sostituita senza danneggiare il ciclo litico del fago. Replicazione del DNA del fago l durante il ciclo litico ed il ciclo lisogenico. Forma replicativa. Lambda phage vectors

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Cosmids : Plasmids that contain l cos sites.

Cosmids : Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments; 35-45 kb. PACs : Based on P1 bacteriophage. Replicate as low copy number plasmids inside E. coli.

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