'Dna binding' diaporamas de présentation

CHAPTER 28 (Genes X) EUKARYOTIC TRANSCRIPTION REGULATION

CHAPTER 28 (Genes X) EUKARYOTIC TRANSCRIPTION REGULATION. Control of Gene Expression in Eukaryotes Eukaryotic gene expression is usually controlled at the level of initiation of transcription. the promoter. enhancers. the TATA box. silencers. GC box. Methylation. CCAAT box

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RNA2: Last week's take home lessons

RNA2: Last week's take home lessons. Clustering by gene and/or condition Distance and similarity measures Clustering & classification Applications DNA & RNA motif discovery & search. Protein1: Today's story & goals. Protein interaction codes(s)? Real world programming

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p53 - guardian or the genome

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Regulation of gene expression

Regulation of gene expression. Same DNA ? complex organism human embryo. Same DNA ? diverse features. Highly similar DNA. Human & Chimpanzee DNA: 96% identical. Regulation of protein level in cell. 1. Synthesis of the primary RNA transcript ( transcription )

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bZIP: leucine zippers

bZIP: leucine zippers. Leucine-zipper (bZIP) -family: common DBD-structure. 60-80 aa motif found in many dimeric TFs Prototypes: GCN4, Fos, Jun, C/EBP, ATF, CREB several possible dimer-partners ? numerous combinations rapid equilibrium ? combinations determined by abundance

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Biophysics 101 Genomics and Computational Biology

Biophysics 101 Genomics and Computational Biology. Schedule: Tue Sep 19 DNA 1: Life & computers; comparative genomics, databases; model utility Tue Sep 26 DNA 2: Polymorphisms, populations, statistics, pharmacogenomics

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ChIP-Seq

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Protein Nucleic Acid Interactions

Protein Nucleic Acid Interactions. September 28, 2006. Different types of recognition motifs. Helix turn helix Homeodomains Zinc finger Leucine zipper Winged helix Beta ribbon. 1. Helix turn helix HTH. Most common DNA binding motif Procaryotes Found in hundreds of DNA binding proteins

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Gene Regulatory

By Fatchiyah (fatchiya@ub.c.id) Dept. of Biology, Brawijaya University. Gene Regulatory. Regulation of Genes. What turns genes on (producing a protein) and off? Where (in which cells) is a gene turned on? When is a gene turned on or off?

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Transcription as a Permutation Algorithm

Transcription as a Permutation Algorithm. By M. Nickenig Mentor: Prof. Robert Vellanoweth. Transcription: Overview. Where does transcription fit into the larger genetic schema: DNA ------------> DNA --------------> mRNA ------------> Protein.

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Section N Regulation of Transcription in Eukaryotes

Section N Regulation of Transcription in Eukaryotes. N1 Eukaryotic Transcription Factors N2 Examples of Transcriptional Regulation. N1 Eukaryotic Transcription Factors. Structure of a typical eukaryotic gene Transcription factor domain structure DNA-binding domains

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Chromosomes and DNA Replication

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Introduction to Epigenetics

Introduction to Epigenetics. BMI/CS 776 www.biostat.wisc.edu/bmi776/ Spring 2019 Colin Dewey colin.dewey@wisc.edu. These slides, excluding third-party material, are licensed under CC BY-NC 4.0 by Anthony Gitter, Mark Craven, and Colin Dewey. Goals for lecture. Key concepts

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The lac Operon Laboratory

The lac Operon Laboratory. Purpose: Learning to analyze an example of gene expression Example: lac operon Type of control: negative inducible. The Laboratory Worksheet asks: How do mutations affect gene expression of the lac operon?. Review: Lac Operon:.

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Electro Mobility Shift Assay EMSA Band Shift Assay Gel Shift Assay

Electro Mobility Shift Assay EMSA Band Shift Assay Gel Shift Assay. R. Voll 09/01. Gene Regulation by Transcription Factors. Coding Sequence. Regulatory Region. R. Voll 09/01. Application:. Detection of DNA-binding factors/proteins

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INDM 3007

INDM 3007. Lecture 9. Proteins interact with DNA: how do they know where to bind?. DNA appears to a homogenous molecule, no specific features to recognise is this true?. What is unique to a particular stretch of DNA? Local shape of DNA Nucleotide sequence.

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Modular Structure of Transcription Factors

Modular Structure of Transcription Factors.

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Regulation by the Heme Cofactor in Proteins

Regulation by the Heme Cofactor in Proteins. Robert W. Clark Visiting Assistant Professor Chemistry clarkr@hope.edu. B.S., Central Michigan University, 1999 M.S., University of Wisconsin-Madison, 2001 Ph.D., University of Wisconsin-Madison, 2005. Heme-specific inhibition

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