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MB206-Jan09

MB206-Jan09. Project. Samples : Plant (A) . Objective: Isolate 100 ESTs from Plant (A). RNA Extraction. Extract RNA from sample (A) – method depend on sample. Check previous note. Check the quality and quantity of the RNA. Isolate mRNA from the RNA (using kits)

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PCR and qRT-PCR

PCR and qRT-PCR. May 3. PCR. The thermocycle analyzing the products essential components of the reaction optimization basic rules of primer design problems with contamination - false positives. Essential components of the reaction. template (DNA or RNA -&gt;DNA)

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278 views • 9 slides

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