E6ap - PowerPoint PPT Presentation

Making Directed Libraries using Rosetta

Making Directed Libraries using Rosetta. Gurkan Guntas (Kuhlman Lab). PROBLEM E6AP has several natural binding partners including UbcH7. Most partners have a crucial phenylalanine at the interface. The crystal structure of E6AP-UbcH7 complex has been solved.

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HEK alone

S1. C/EBP a and E6AP co-localize in the nucleus. HEK alone. HEK+C/EBP α. C/EBP α + E6AP 1.0 μ g. C/EBP α + E6AP 2.0 μ g. Merge. DAPI. E6AP. C/EBP α. S2. GST-E6AP Purification. GST-E6AP+IPTG. GST alone. GST-E6AP. Marker. 1 2 µg(BSA). GST-E6AP. BSA. GST. S3a.

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209 views • 6 slides

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