Elution volume - PowerPoint PPT Presentation

Chapter 26

Chapter 26. Other Methods. Ion-Exchange Chromatography. The mechanism of separation will be the exchange of ions from the column to the solution. Water softening – exchange Na ions for Ca and Mg. Water deionization – exchange H ions for cations and OH ions for anions. Leaving water.

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Biochemistry 412 2004 20 February Lecture Analytical &amp; Preparative Protein Chemistry II

Biochemistry 412 2004 20 February Lecture Analytical &amp; Preparative Protein Chemistry II. Proteins are Amphiphilic Macro-Ions. Positively-charged basic residues (K, R, &amp; H). Hydrophobic “patch”. Macromolecular dimensions:. ca. 40 Å. Ligand binding pocket (active site).

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HPLC

HPLC. Lecture 42. Mobile Phase Selection in Partition Chromatography.

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670 kD. 158 kD. 44 kD. 17 kD. 1.35 kD. mAU. 8.0. 6.0. 4.0. UV absorption. 2.0. 0.0. ml. 0.0. 5.0. 10.0. 15.0. 20.0. Elution volume.

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Elution volume - PowerPoint PPT Presentation

Chapter 26

Biochemistry 412 2004 20 February Lecture Analytical &amp;amp; Preparative Protein Chemistry II

HPLC

0.0

Biochemistry 412 2004 20 February Lecture Analytical & Preparative Protein Chemistry II