Est databases - PowerPoint PPT Presentation

In-Gel Digestion Why In-Gel Digest? Difficult / impossible to extract intact proteins from the gel

In-Gel Digestion Why In-Gel Digest? Difficult / impossible to extract intact proteins from the gel Generate peptides that facilitate Protein ID Enzymes? Trypsin, Trypsin, Trypsin…--Cleaves after Arg (R) &amp; Lys (K) Lys C--Cleaves after Lys (K) V8-- (Cleaves after Asp (D) &amp; Glu (E)

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1.12k views • 17 slides

In-Gel Digestion Why In-Gel Digest? Difficult / impossible to extract intact proteins from the gel

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442 views • 17 slides

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