Ethanol precipitation - PowerPoint PPT Presentation

Theophylline Responsive Riboswitches

Theophylline Responsive Riboswitches. Becca Evans Summer 2012. Aptamers. Short nucleic acid sequence that binds to a specific small molecule or ligand. Riboswitch. Translational control Contains aptamer sequence In 5’ untranslated region of mRNA. GOI: Translated Region.

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Objectives of our project

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Isolation of Nucleic Acids

Isolation of Nucleic Acids. Goals : removal of proteins DNA vs RNA isolation of a specific type of DNA (or RNA). Types of Methods : differential solubility ‘adsorption’ methods density gradient centrifugation. Types of DNA : genomic (chromosomal) organellar (satellite)

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963 views • 22 slides

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