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Methods in Molecular Biology

Methods in Molecular Biology

Molecular Biology Bio4751 Spring 2003 Gary A. Bulla, PhD. Methods in Molecular Biology. RNA quantitation. a. Northern. 1. Lyse with detergent, 2. Phenol extract (removes proteins) 3. Precipitate RNA 4. Load onto agarose gel 5. Transfer RNA to nylon membrane

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(233 views)


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DNA-PROTEIN INTERACTIONS

DNA-PROTEIN INTERACTIONS

DNA-PROTEIN INTERACTIONS. AN IN-CLASS PROJECT. DNA-PROTEIN INTERACTIONS. NONSPECIFIC LOOSE BETWEEN PROTEIN FUNCTIONAL GROUPS AND DNA BACKBONE IONIC H-BONDS VANDERWAALS SPECIFIC (SEQUENCE-SPECIFIC) TIGHT BINDING DIRECT H-BONDING INDIRECT H-BONDING “INDIRECT READOUT”.

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DNA-PROTEIN INTERACTIONS

DNA-PROTEIN INTERACTIONS

DNA-PROTEIN INTERACTIONS. AN IN-CLASS PROJECT. DNA-PROTEIN INTERACTIONS. NONSPECIFIC LOOSE BETWEEN PROTEIN FUNCTIONAL GROUPS AND DNA BACKBONE IONIC H-BONDS VANDERWAALS SPECIFIC (SEQUENCE-SPECIFIC) TIGHT BINDING DIRECT H-BONDING INDIRECT H-BONDING “INDIRECT READOUT”.

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DNA & DNA : Protein Interactions

DNA & DNA : Protein Interactions

DNA & DNA : Protein Interactions. BIBC 100. Sequence = Information. Alphabet = language L,I,F,E LIFE DNA = DNA code A, T, C, G CAC=Histidine CAG=Glutamine GGG=Glycine Protein = Protein code 20 a.a. L, I, F, E N C NC. LIVE EVIL. DNA basics.

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Detecting DNA-protein Interactions

Detecting DNA-protein Interactions

Detecting DNA-protein Interactions. Xinghua Lu Dept Biomedical Informatics BIOST 2055. DNA and Proteins. DNA-protein Interactions of Interest. The ENCODE Project. ENCODE. An Overview of Technologies. Study the interaction of specific DNA sequences and specific proteins Gel shift assay

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In vivo protein-DNA interactions

In vivo protein-DNA interactions

In vivo protein-DNA interactions. Giacomo Cavalli. Institut de Génétique Humaine, CNRS Montpellier, France. UE Méthodologie, 11 April, 2014. Compaction by higher order determinants. 10,000 nm. DNA compaction in the nucleus. 11 nm. 30nm. 1bp (0.3nm). Compaction of DNA by histones.

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DNA-Protein Interactions & Complexes

DNA-Protein Interactions & Complexes

DNA-Protein Interactions & Complexes. Prokaryotic promoter. Consensus sequence is not present in majority of prokaryotic promoters . Sequence motifs encompassing 6-10 nucleotides are degenerate; Probability of finding similar sequences in regions other than promoters is quite high. 6.

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Chapter 12: DNA-protein interactions

Chapter 12: DNA-protein interactions

Chapter 12: DNA-protein interactions. Preparation of nuclear extracts and cytoplasmic (S-100) fraction Use hypotonic solutions and 10 up-and-down strokes with a glass Dounce homogenizer. Check for cell lysis under a microscope and trypan blue exclusion.

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Computational Analysis of Protein-DNA Interactions

Computational Analysis of Protein-DNA Interactions

Computational Analysis of Protein-DNA Interactions. Changhui (Charles) Yan Department of Computer Science Utah State University. Problem I. Identifying amino acid residues involved in protein-DNA interactions from sequence. Materials And Methods.

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Protein/DNA Interactions and Gene Expression

Protein/DNA Interactions and Gene Expression

Protein/DNA Interactions and Gene Expression. Michael J. Pikaart Assistant Professor Chemistry pikaart@hope.edu. Photograph. B.S., Calvin College, 1986 Ph.D., University of Michigan, 1992. Areas of expertise: Biochemistry, Transcription regulation, Chromatin structure. Grants and awards

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Protein – Protein Interactions

Protein – Protein Interactions

Protein – Protein Interactions. Implementation/Algorithm. Algorithm approximates the minimum set of domains pairs. Algorithm needs to be able to choose d-d pairs in an educated, not a randomized fashion.

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