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By Thomas J. Schreiber. TERMINATOR. In a post-apocalyptic 2029, artificial intelligent machines seek to exterminate what is left of the human race. Possibility?.
John Connor, leader of the human resistance against Skynet, sends Kyle Reese back to 1984 to protect his mother, Sarah and the complete film revolves around her, from a Terminator assassin, an unexpected turn of events creates an altered time-line. Instead of a scared waitress, Sarah is a skilled fighter and has a Terminator guardian, Arnold Schwarzenegger. Faced with unlikely allies and dangerous new enemies, Reese sets out on an unexpected new mission: reset the future.
Fig. S1. Pst I digestion + ligase. Pst I. Pst I. T7-TopRI-5. T7-TopRI-4. Pst I. Pst I. Pst I. Pst I. PCR 4 + 5. T T7. T T7. Bam HI. Bgl II. pET-9d. T7 terminator. T T7. P T7. ATG. pQET7-TopRI. megaprimer. T7-TopRI-3. Col E1. Sac I. Xho I. Sac I. Xho I. PCR 3 + mega.
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