Fha domain - PowerPoint PPT Presentation

PLAPA

PLAPA. Phosphate Localization And Prediction Algorithm. How does it work?. Generates a surface using Matt’s surface generation tool in PLOP Places a phosphate “ball” of -2 charge at each point Minimizes the position of the ball Calculates energy

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522 views • 39 slides

Example: Critical Evaluation of a Paper

Example: Critical Evaluation of a Paper. Barthe et al. (2009) Dynamic and Structural Characterization of a Bacterial FHA Protein Reveals a New Autoinhibition Mechanism. Structure , 17:568-578. Major Observations &amp; Conclusions: OdhI protein is key regulator of the TCA cycle

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233 views • 9 slides

Bam HI

A. XbaI. 10xHis. FHA domain. BamHI. Phosphate domain. Kinase domain. Bam HI. 5’-. -3’. C. F10. B. Lysate. FL. W1. W2. F1. F2. F3. F4. F5. F6. F7. F8. F9. 62. Bam HI/XbaI. Bam HI. Marker. 47.5. 32. 10..0K. 25. 8.0K. 6.0K. 5.0K. 4.5K. 16.5. 4.0K. 3.5K. 3.0K.

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121 views • 1 slides

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