Fluorescence correlation spectroscopy - PowerPoint PPT Presentation

Jerker Widengren Exp. Biomol. Physics Dept. P hysics , KTH

Detection, identification and conformational dynamic characterization of single molecules by ultra-sensitive fluorescence spectroscopy techniques. Jerker Widengren Exp. Biomol. Physics Dept. P hysics , KTH. Topics of Discussion. F luorescence Correlation Spectroscopy (FCS) - Concept

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FCS: Fluorescence Correlation Spectroscopy

FCS: Fluorescence Correlation Spectroscopy. A Confocal Microscope. Can illuminate and detect fluorescence in a tiny volume &lt;0.5 um in width. &lt;F&gt;. F(t). ω xy. 2 ω z. FCS allows observations of diffusion in living cells and different parts of living cells.

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761 views • 8 slides

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