'Foreign dna fragment' diaporamas de présentation

19.1 Techniques of Molecular Genetics Have Revolutionized Biology

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Chapter 13

Chapter 13. Recombinant DNA: Cloning and Creation of Chimeric Genes to accompany Biochemistry, 2/e by Reginald Garrett and Charles Grisham.

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DNA Recombinant Technology

DNA Recombinant Technology. What and Why?. What?: A gene of interest is inserted into another organism, enabling it to be cloned, and thus studied more effectively

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13.1 Applied Genetics

13.1 Applied Genetics . p337. Selective breeding. Selecting specific traits Best chickens, juiciest fruit Increasing the frequency of desired alleles in a population is genetic technology. Inbreeding. Mating between closely related species. Used to get desired traits Purebreds

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The small sample of DNA serves as template for DNA polymerase Make complementary primers Add primers in more than 1000

The Polymerase Chain Reaction. The small sample of DNA serves as template for DNA polymerase Make complementary primers Add primers in more than 1000-fold excess Heat to make ssDNA, then cool Run DNA polymerase (usually Taq) Repeat heating, cooling, polymerase cycle.

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Cloning Genes

Cloning Genes. Gene cloning: amplifying a specific piece of DNA via a bacteria cell Cloning vector: a replicating DNA molecule attached with a foreign DNA fragment to be introduced into a cell Has features that make it easier to insert DNA and select for presence of vector in cell.

1.48k views • 11 slides