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Contents (1)

Contents (1)

Molecular Evolution of Proteins and Phylogenetic Analysis Fred R. Opperdoes Christian de Duve Institute of Cellular Pathology (ICP) and Laboratory of Biochemistry, Université catholique de Louvain, Brussels, Belgium. Contents (1).

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1.37k views • 117 slides
Abdelilah Soussi Gounni, Ph.D Associate Professor Manitoba Research Chair

Abdelilah Soussi Gounni, Ph.D Associate Professor Manitoba Research Chair

Quantification of mRNA using real-time PCR. Abdelilah Soussi Gounni, Ph.D Associate Professor Manitoba Research Chair Department of Immunology University of Manitoba. Objectives Introduction to qPCR Chemistries used in qPCR Different platforms : pro and con

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815 views • 63 slides
Bacterial Agents of Disease

Bacterial Agents of Disease

Bacterial Agents of Disease. Gram-Positive Group. Traditionally, this group was based on Gram stain procedure Now the grouping is based on Genetic similarity Group is divided into two taxa based on GC content High GC Gram positives (>50%) Low GC Gram positives (<50%)

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743 views • 56 slides
What have we learned from Unicellular Genomes?

What have we learned from Unicellular Genomes?

What have we learned from Unicellular Genomes?. Propionibacterium acnes. Responsible for acne, its genome sequenced in 2004. It lives on human skin in sebaceous follicles; feeds on sebum and this stimulates immune response of inflammation. Can we understand pimples?. Anatomy of acne.

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646 views • 55 slides
Amplifying DNA: PCR & cell-based DNA cloning

Amplifying DNA: PCR & cell-based DNA cloning

Amplifying DNA: PCR & cell-based DNA cloning. The importance of DNA cloning: Current DNA technology is based on two different approaches:

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1.61k views • 53 slides
The biology of Leishmania beyond the Genome Project

The biology of Leishmania beyond the Genome Project

The biology of Leishmania beyond the Genome Project. Leishmania life cycle. H u m a n. S a n d f l y. Leishmania : some features. No chromosome condensation Generally accepted as a diploid, asexual organism No mutants available. A Friendly parasite for genome research

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556 views • 39 slides
Welcome to Molecular Biology Through Discovery Tuesday, 25 September 2012 Benzer (1959)

Welcome to Molecular Biology Through Discovery Tuesday, 25 September 2012 Benzer (1959)

Welcome to Molecular Biology Through Discovery Tuesday, 25 September 2012 Benzer (1959). General Questions. What would you like discussed in class, on any subject that has already come up?. I would like to learn more about how to use probability formulas when dealing with DNA frequencies.

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454 views • 28 slides
Biological Motivation Gene Finding

Biological Motivation Gene Finding

Biological Motivation Gene Finding. Anne R. Haake Rhys Price Jones. Gene Finding. Why do it? Find and annotate all the genes within the large volume of DNA sequence data how many genes in an organism? homologies? Gain understanding of problems in basic science

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429 views • 27 slides
Sorting and Modules

Sorting and Modules

Sorting and Modules. Sorting. Lists have a sort method. Strings are sorted alphabetically, except. >>> L1 = ["this", "is", "a", "list", "of", "words"] >>> print L1 ['this', 'is', 'a', 'list', 'of', 'words'] >>> L1.sort() >>> print L1 ['a', 'is', 'list', 'of', 'this', 'words'] >>>

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323 views • 24 slides
Comp. Genomics

Comp. Genomics

Comp. Genomics. Recitation 7 2/4/09 PSSMs+Gene finding. Partially based on slides by Irit Gat-Viks and Metsada Pasmanik-Chor. Biological Motifs. Biological units with common functions frequently exhibit similarities at the sequence level. These include very short “motifs”, such as:

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366 views • 23 slides
Advanced Perl For Bioinformatics

Advanced Perl For Bioinformatics

Advanced Perl For Bioinformatics. Part 1 2/23/06 1-4pm Module structure Module path Module export Object oriented programming Part 2 2/24/06 1-4pm Bioperl modules Sequence access Sequence manipulation Parsing BLAST records. Module and main program. package Hello1; sub greet {

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427 views • 22 slides
Basic Molecular Biology Information

Basic Molecular Biology Information

Basic Molecular Biology Information. Central Dogma of Molecular Biology. Concerns the flow of information in the cell. Nucleic Acids. Only 2 types: DNA and RNA linear chains of nucleotides DNA: 2 chains running anti-parallel twisted together into a double helix

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452 views • 21 slides
Natalia Ivanova

Natalia Ivanova

Metagenome analysis: use case. Natalia Ivanova. MGM Workshop September 29, 2011.

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238 views • 21 slides
Group 6

Group 6

Group 6. Bikash Shakya Emma Lang Jorge Diaz. Sequence 6. BLASTx entire sequence against 9 plant genomes. RepeatMasker  55.47 %  repetitive  sequences 82.5 % retroelements 13.0 % DNA transposons EMBOSS explorer 74 CpG islands 54 inverted repeats. GENE PREDICTION.

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351 views • 18 slides
LAB 5: IDENTIFICATION OF PATHOGENIC BACTERIA BY PCR AMPLIFICATION OF THE 16s rRNA GENE & RESTRICTION FRAGMENT

LAB 5: IDENTIFICATION OF PATHOGENIC BACTERIA BY PCR AMPLIFICATION OF THE 16s rRNA GENE & RESTRICTION FRAGMENT

LAB 5: IDENTIFICATION OF PATHOGENIC BACTERIA BY PCR AMPLIFICATION OF THE 16s rRNA GENE & RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP). OBJECTIVES. Describe process & principles of PCR technique Describe how the 16 S rRNA can be used in bacterial identification

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783 views • 17 slides
Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar. Nucleotide Structure: Nitrogenous Bases. DNA Linkage. Phosphate group/phosphodiester bond Nucleotides contain one, two, or three phosphate groups. *nucleoside *nucleotide. Nucleic Acid Polarity. *Top of molecule has free 5’-phosphate group

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358 views • 11 slides
GABI-KAT system

GABI-KAT system

GABI-KAT system

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255 views • 10 slides
Deinococcus radiodurans

Deinococcus radiodurans

Deinococcus radiodurans. Presentation by: Monica Tydlaska. Why this species?. Found in diverse environments Like elephant dung and granite in Antarctic dry valleys No real knowledge of real habitat exists It has the ability to survive a thousand times more radiation than a person could

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489 views • 9 slides
Brückner et al., Fig. 1b

Brückner et al., Fig. 1b

Brückner et al., Fig. 1b. 1. 2. 3. 12. 4. c. b. 11. 5. a. 10. 6. 9. 7. 8. Brückner et al., Fig. 1B.

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140 views • 4 slides
log2(frequency ratio)

log2(frequency ratio)

A. B. Difference in GC content. Difference in GC content. C. D. log2(frequency ratio). log2(frequency ratio). Difference in GC content. Difference in GC content. log2(frequency ratio). log2(frequency ratio). E. F. Difference in GC content. Difference in GC content.

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87 views • 1 slides

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