'Gfp green fluorescent protein' diaporamas de présentation

Problem

Problem. 1. You screen two libraries- cDNA; genomic 2. Clones are isolated having homology to PSY- 10 clones from each library 3. These are subcloned into pBluescript. 4. Protein expression is induced with IPTG and proteins separated by SDS-PAGE. Results: Genomic clones: 0/10 gave expression

530 views • 33 slides

Way to Glow! Bacterial Transformation

Way to Glow! Bacterial Transformation. Transformation of E. coli with pFluoroGreen. What is our goal?. To express green fluorescent protein ( GFP) into transformed bacterial cells. Laboratory Safety. Gloves Goggles Lab coat Non-pathogenic strain of E. coli. Transformation of E. coli.

975 views • 28 slides

Way to Glow! Bacterial Transformation

Way to Glow! Bacterial Transformation. Transformation of E. coli with pFluoroGreen. What is our goal?. To express green fluorescent protein ( GFP) into transformed bacterial cells. Laboratory Safety. Gloves Goggles Lab coat Non-pathogenic strain of E. coli. Transformation of E. coli.

688 views • 28 slides

GFP ( Green fluorescent protein) by Kitija Kaulina

353 views • 18 slides

RFP Transformation Lab

RFP Transformation Lab. Images taken without permission from http://web.aibn.uq.edu.au/cbn/research_biomolecular.htm , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg. Overall Goal of Lab Experiment.

331 views • 16 slides

pGLO Transformation LAB AP LAB 6

758 views • 15 slides

C. elegans as a model organism

C. elegans as a model organism. Kavya Leo Vakkayil 20101080, BS-MS IISER PUNE. GROWTH OF A RESEARCH AREA. Jonathan Hodgkin, University of Oxford. 1. An introduction to C. elegans Anatomy Life cycle 2. A short history of C. elegans research

925 views • 15 slides

GFP Transformation Lab

GFP Transformation Lab. Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg.

230 views • 14 slides

GFP Transformation Lab

GFP Transformation Lab. Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg.

563 views • 14 slides

Creating Recombinant DNA

254 views • 14 slides