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Gradient gels - PowerPoint PPT Presentation


Buffers

Buffers

Buffers. pH measurement. Voltage  log [H + ] inside /[H + ] outside. Buffers. Buffering capacity Phosphate, carbonate, Tris buffers Good buffers. Electrophoresis. Bible: Gel Electrophoresis of Proteins: Practical Approach, Hammes and Rickwood, IRL Press Principles

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874 views • 15 slides



Protein gel electrophoresis • used to separate proteins based on a number of characteristics Denaturing gel electrophore

Protein gel electrophoresis • used to separate proteins based on a number of characteristics Denaturing gel electrophore

Protein gel electrophoresis • used to separate proteins based on a number of characteristics Denaturing gel electrophoresis separate by size Nondenaturing (native) gel electrophoresis separate by size, shape, charge. Protein gel electrophoresis Polyacrylamide gel electrophoresis.

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557 views • 13 slides


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