'Homologous recombination' diaporamas de présentation

Objectives of DNA recombination

Objectives of DNA recombination. The different processes of DNA recombination: homologous recombination, site-specific recombination, transposition, illegitimate recombination, etc.

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Allele

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JEOPARDY

JEOPARDY. Answer. A dense region within the bacterial cell where the chromosome is found. Question. What is the nucleoid region ?. Answer. In the true bacteria this is an essential component of the cell wall. Question. What is peptidoglycan?. Answer.

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Chapter 10 Recombination / 重组

Chapter 10 Recombination / 重组. 10.1 同源重组 10.1.1 交换机理 10.1.2 双链断裂 修复机理 10.1.3 RecBCD 途径 10.1.4 基因转换 10.2 非同源重组 10.2.1 转座子 10.2.2 反转录转座子 10.2.3 λ 噬菌体的整合. 10.1 Homologous Recombination 10.1.1 Mechanism for Crossing-Over

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Snapshot of the next four weeks

BEH.109: Laboratory Fundamentals in Biological Engineering. MODULE 3 Eukaryotic Cells as Phenotypic Indicators: The use of RNAi to modulate gene expression DAY 3. Snapshot of the next four weeks

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Genetic Recombination

Purposes Promotes genetic diversity within a species - within a chromosome causes inversions, deletions, duplications - horizontal exchange introduces new sequences (information) . In the lab: . introduce foreign DNA or mutations into bacteria .

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Molecular biology

Molecular biology. Transformation: introduction of DNA Selectable marker Spheroplasts, Li 2+ salts, electroporation Yeast plasmids are shuttle plasmids, amplification in E. coli, ori, b -lactamase Transformation with oligonucleotides, -> selection

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DNA Recombination

DNA Recombination. Roles Types Homologous recombination in E.coli Transposable elements. Biological Roles for Recombination. Generating new gene/allele combinations (crossing over during meiosis) Generating new genes (e.g., Immuno- globulin rearrangement)

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Lambda RED Recombination

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Chapter 10

Chapter 10. Homologous Recombination at the Molecular Level ----- ??? ?? 200431060171. OUTLINE. Models for Homologous Recombination( two models) ? Homologous Recombination Protein Machines ? Homologous Recombination in Eukaryotes Mating-Type Switching

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PMID:15889139

PMID:15889139. Mutations in two, or more, genes. IGI. Biological Process:. meiosis?. Mutations in a single gene. IMP. homologous recombination?. Physical interactions between proteins. IPI. with:. SGS1. TOP3. Cellular Component:. complex involving Sgs1p, Top3, and Rmi1p. IMP.

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Regulation of Virulence Genes

Regulation of Virulence Genes. Salyers & Whitt: Bacterial Pathogenesis: A Molecular Approach ASM Press, 1994 Dorman, C.J: Genetics of Bacterial Virulence. Blackwell Scientific Publications, 1994. Virulence Factors. Invasion Penetration Protection Nutrition Dissemination. Overview.

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??????? CRISPR- Cas ?????

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Mechanism of Homologous Recombination

Mechanism of Homologous Recombination. MCB 720 Yahan wei. Outline. Background information Mechanisms of Homologous Recombination (HR) Reference. What is HR?. Exchange of nucleotide sequences between two similar or identical DNA molecules

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Lecture 25: DNA repair, recombination; changes in chromosome number

Lecture 25: DNA repair, recombination; changes in chromosome number. Non-homologous end joining repairs DSBs. DSB repair by homologous recombination. Sister chromatids. Initiation of meiotic recombination. Recombination: Holliday junctions. Holliday junction. Gene conversion.

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killer vegetables, animal-human hybrids, other scary stuff.

killer vegetables, animal-human hybrids, other scary stuff. KEVIN HIOM. Galway 2010. Chapter 1: Epistasis for beginners. Basic principles of DT40. DT40: A genetically tractable eukaryotic cell line. DT40. Genetically tractable Good model for genome stability in mammals

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ChapTer 20: Human Evolution

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Sharing of genetic information

Sharing of genetic information. Bacteria are successful because They carefully regulate their use of energy in metabolic processes by shutting down unneeded pathways at the biochemical and genetic levels.

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Meiosis

Meiosis. John Ireland, Ph.D. Topics to Cover . Sexual Reproduction Features of Meiosis Meiosis Meiosis vs. mitosis. Sexual Reproduction. Meiosis allows for a reduction of the number of chromosomes in sex cells Germ-line cells (haploid) are set aside and produced through meiosis.

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MH Yun & K Hiom Nature 000 , 1-4 (2009) doi:10.1038/nature07955

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