pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP). Instructors. Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School
By shengPurification of Green Fluorescent Protein (GFP). Instructors. Stan Hitomi Coordinator – Math & Science Principal – Alamo School San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School
By zoranapGLO™ Transformation and Purification of Green Fluorescent Protein (GFP). Workshop Time Line. Introduction Transform bacteria with pGLO plasmid Purify GFP using column chromatography. DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. Links to Real-world.
By maximepGLO ™ Transformation and Purification of Green Fluorescent Protein (GFP). DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. Links to Real-world. GFP is a visual marker Study of biological processes (example: synthesis of proteins)
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GFP GFP-WT GFP-NES. CE NE CE NE CE NE. A. GFP-WT Annexin II. GFP. CE CE NE NE. GFP-NES mutant annexin II. B. PGK. PACP - + - +. 1 2 3 4 5 6. GFP-NES. C.
Gene correction in trans: a model for gene therapy. gfp -. Luther Davis Jason Cummings Nancy Maizels. Gene correction in trans: a model for gene therapy. GFP +. Luther Davis Jason Cummings Nancy Maizels. nuclease. Gene correction in trans. Cut sites. eGFP. Poly-lacO. Puro. trGFP.
A. MSCV. MSCV. GFP-RV. 5 ‘ LTR. IRES. GFP. 3 ‘ LTR. 3 ‘ LTR. mIFN- . IRES. GFP. IFN/GFP-RV. 5 ‘ LTR. B. GFP-RV IFN/GFP-RV. 35%. 34%. 55%. 56%. CD 8. 2%. 2%. 8%. 8%. GFP. C. GFP-RV IFN/GFP-RV. 99%. 99%.
A. B. 5 ’- LTR. U6.2. shRNA. CMV. GFP. shRNA CONSTRUCT Scrambled GGATCCC GACGATTCGAGGCGCAGTGAT TTGATATCCG ATCACTGCGCCTCGAATCGT CTTTTTTCCAACTCGAG Cyp2b-KD2 GGATCCC AAGAACACTGAGGTGTACCCC TTGATATCCG GGGGTACACCTCAGTGTTCTT TTTTTTCCAACTCGAG
A. Plasmid 1 GCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCTCGAAATTGTAAAAAGCGGACCG Full-length 1 GCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCTCGAAATTGTAAAAAGCGGACCG
T 0. T 8. Untreated. G 1. 46%. 13%. 46%. S. 19%. 11%. 17%. G 2. 35%. 76%. 37%. T 0. T 8. Untreated. G 1. 44%. 8%. 45%. S. 14%. 6%. 16%. G 2. 42%. 86%. 39%. T 0. T 8. Untreated. G 1. 43%. 10%. 46%. S. 16%. 8%. 10%. G 2. 41%. 82%. 44%. GFP. A. B. GFP.
A. Van der Gun et al. B. pMX. ZF A SKD. ZF B VP64. EpCAM. GFP. Supplementary: Figure 1 Bidirectional EpCAM expression modulation. D. Van der Gun et al. C. E. Supplementary: Figure 1 Bidirectional EpCAM expression modulation.
GFP. Discovery and First Applications. Discovery of GFP protein. 1960: Osamu Shimomura began studying bioluminescence in Aequorea victoria. 1962: Reported properties of aequorin , a protein which glows blue around Ca 2+ ions