Ionizable groups - PowerPoint PPT Presentation

Lecture 7: Protein purification

Lecture 7: Protein purification. Protein purification. Methods of solubilization. 1st step is to get the protein into solution If the protein is located in the cytosol, we need to break open the cell.

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Coulomb interactions between internal ionizable groups and surface residues

Coulomb interactions between internal ionizable groups and surface residues. Victor Khangulov May 13, 2009 Institute in Multiscale Modeling of Biological interactions Johns Hopkins University Laboratory of Dr. Garcia-Moreno.

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