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IRS1-pSOS + HDAC2-pMyr. PH. PTB. Pre-C. C-term. IRS-1pSos + pMyr. pSos + HDAC2-pMyr. PH. PTB. Pre-C. C-term. 1 2 3. 191. IRS-1. f.l. HDAC2. Bait. 64. -. PH. PTB. HDAC2. 28. +. PH. PTB. Pre-C. +. PH. +. +. A. B. C.
NGC7538-IRS1 N: modelling a maser disc. Michele Pestalozzi, M. Elitzur, J. Conway, R. Booth. Discs in high-mass star-forming regions. Zhang et al. 1998 Cesaroni et al. 2000 Shepherd et al. 2001 Sandell et a l. 2003. NGC7538-IRS1N: “the” disc. 6.7 GHz. Pestalozzi et al. 2004.
Protein stability, protein-protein interactions. 11-1. Protein stability - how proteins achieve stability under extreme conditions (class presentation) Protein-protein interactions - recent literature (presentations). Stabilization/destabilization forces affecting proteins. 11-2.
Protein protein interactions. Better understanding of protein b’s function. Introduction. Protein b unknown. Protein a known. Interaction. Detecting relationship between pathways. Detecting new pathways. Introduction. Protein a Function a. Protein b Function b. Interaction.
Protein-protein interactions. Chapter 12. Stable complex: homodimeric citrate synthase. Transient Signaling Complex Rap1A – cRaf1. Multi-domain protein. Interface 4890 Å 2. Interface 1310 Å 2. Stable vs . transient protein-protein interactions. Transient Interaction. Stable complex.
Protein-protein interactions. Masoud Youssefi, MD,PhD Division of microbiology/virology. Introduction. important field in cell biology, biochemistry Localization and trafficking posttranslational modifications signaling networks also important field in viral replication
Protein – Protein Interactions. Implementation/Algorithm. Algorithm approximates the minimum set of domains pairs. Algorithm needs to be able to choose d-d pairs in an educated, not a randomized fashion.
Protein-Protein Interactions. Protein Interactions. A Protein may interact with: Other proteins Nucleic Acids Small molecules. Protein-Protein Interactions: The “ Interactome ”. Experimental methods: Mass Spec, yeast 2-hybrid system, microarrays,… Computational techniques:
Protein-protein Interactions. Outline. Introduction The Study of Protein-protein Interaction by Mass Spectrometry Yeast-Two-Hybrid Phage Display Protein-protein Interaction on the Web Information Extraction (IE). Introduction The Study of Protein-protein Interaction by Mass Spectrometry
Protein-Protein Interactions. Genome. Transcriptome. Proteome. Interactome. Atomic NMR spectroscopy X-ray crystallography: co-crystallisation Electron microscopy Molecular modelling: docking Protein Data Bank. Experimental methods to identify PPI’s. Biochemical/biophysical binary