Loop insertion - PowerPoint PPT Presentation

Cling- E. coli : Bacteria on target

Cling- E. coli : Bacteria on target. Harvard iGEM 2007 Ellenor Brown Stephanie Lo Alex Pickett Sammy Sambu. Kevin Shee Perry Tsai Shaunak Vankudre George Xu. The motivation. To develop a system for directing bacteria to a target of interest and effecting downstream activity.

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464 views • 34 slides

Slide Collection

Slide Collection. SKS. Techniques. Fusion of ds-synthetic oligos to the C terminus OmpA &amp; AIDA1 Fusion to the N terminus AIDA1 Fusion to extracellular portion of loop 1 ( loop insertion) OmpA. Fusion at C terminus: Bead Assay (His tag). Pre-assay plates. Beads. AIDA results.

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220 views • 12 slides

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