'Mcm2 7' diaporamas de présentation

Lecture 3:

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DNA Replicates by a Semiconservative Mechanism

DNA Replicates by a Semiconservative Mechanism. Grow cells in 15 N and transfer to 14 N. Analyze DNA by equilibrium density gradient centrifugation. Presence of H-L DNA is indicative of semiconservative DNA replication. from Lodish et al ., Molecular Cell Biology, 6 th ed. Fig 4-29.

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DNA Replicates by a Semiconservative Mechanism

DNA Replicates by a Semiconservative Mechanism. Grow cells in 15 N and transfer to 14 N. Analyze DNA by equilibrium density gradient centrifugation. Presence of H-L DNA is indicative of semiconservative DNA replication. from Lodish et al ., Molecular Cell Biology, 6 th ed. Fig 4-29.

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Yeast Has Defined Origins

Yeast Has Defined Origins. ARS directs autonomous replication of plasmid DNA. S. cerevisiae ARS contains a conserved 11 bp ARS consensus sequence and multiple B elements. The ORC complex binds to the ARS during most of the cell cycle. The S. pombe origin is larger and

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???? DNA ?????????

???? DNA ?????????. ???? ?????????????? ??????. ????. DNA ?????. DNA ???. DNA ???? PRIMASE ?. ? DNA ????? RNA ?????????? 2 ?????????????????????????????????????. DNA ???. DNA ?????. ??????? SSB ?. ???? nucleases ?.

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Formation of pre-Replicative complex

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G 1

MCM2-7. CycA. Geminin. ORC. Cdt1. P. Cdc6. S. P. G 2. M. G 1. CycA. Geminin. High APC/C activity. ORC. Cdt1. Cdc6. MCM2-7. High Emi1 levels.

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