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mRNA. Cap Poly(A) tail Secondary structures IRES uORF Protein/RNA binding sites AUG Kozak consensus sequence: GCC( A / G )CC AUG G. Fátima Gebauer & Matthias W. Hentze Nature Reviews Molecular Cell Biology 5, 827-835 (2004). Scanning model of Cap-dependent Translation Initiation. E.
Liver cell cytoplasmic membrane. (a). HBV infects liver cell. Cell nucleus. (b). Polymerase completes DNA strand. (c). Completed viral genome transported to cell nucleus, messenger RNA and RNA copy of genome transcribed. (d). Reverse transcriptase and other viral proteins are
WRN. PAI-1. C. C. C. TNF . TNF . TNF . TGF . TGF . TGF . INS. INS. INS. 25. 20. *. *. 15. mRNA / a ctin mRNA. *. 10. *. 5. 1367 CC / 1074 FF. 1367 CR / 1074 FL. 1367 RR / 1074 FL.
WRN. PAI-1. A. B. Scrambled. iRNA. **. **. 30. 30. 25. 25. 20. 20. **. mRNA / a ctin mRNA. 15. 15. 10. 10. **. 5. 5. *. *. *. *. Control. TNF . TGF . insulin. Control. TNF . TGF . insulin.
EDI. EDII. IIICS. EDI. NH2. COOH. Minigene. 20 splicing isoforms. PAGE. pre-mRNA. EDI. EDI +. RT-PCR. EDI. EDI -. EDI - mRNA. EDI + mRNA. Mu ñoz et al. Figure 1. a. b. Fibronectin. d. c. Mu ñoz et al. Figure 2. 7 tet operators. EDI. a. P hCMV*-1. pUHC-EDA.
mRNA metabolism. Ülo Maiväli ymaivali@ebc.ee. m 7 G CAP lisatakse kõigile RNA pol II produktidele, osadel protsessitakse hiljem maha. mRNA snRNA snoRNA Telomeraasi RNA. CAPi funktsioonid tuumas (kompleksis CBC-ga). mRNA splaisingu aktiveerimine tuumas
mRNA metabolism. CAP. m 7 G CAP lisatakse kõigile RNA pol II produktidele, osadel protsessitakse hiljem maha. mRNA snRNA snoRNA Telomeraasi RNA. CAPi funktsioonid tuumas (kompleksis CBC-ga). mRNA splaisingu aktiveerimine tuumas mRNA 3´-otsa protsessimise/ polüadenülatsiooni aktivatsioon
mRNA Quantification. Real-time PCR. To accurately quantify starting amounts of nucleic acid during the PCR reaction without the need for post-PCR analyses. A fluorescent reporter is used to monitor the PCR reaction as it occurs; the reporter can be of a nonspecific or specific nature.
a. Met. tRNA. i. Leu. tRNA. 10 7. 10 6. c. 10 5. Vec.F9. Brf1.F9. Brf1 mRNA. 10 4. 10 7. b. 10 6. ARPP P0 mRNA. 1. 2. 10 5. 10 4.