Oligonucleotide probe - PowerPoint PPT Presentation

Microarray normalization, error models

Microarray normalization, error models. Wolfgang Huber EMBL. Brief history. Late 1980s : Poustka, Lennon, Lehrach: cDNAs spotted on nylon membranes 1990s : Affymetrix adapts microchip production technology for in situ oligonucleotide synthesis („commercial and heavily patent-fenced“)

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1.07k views • 87 slides

Microarray normalization, error models, quality

Microarray normalization, error models, quality. Wolfgang Huber EMBL-EBI Brixen 16 June 2008. Oligonucleotide microarrays. Base Pairing. *. *. *. *. *. Oligonucleotide microarrays. GeneChip. Hybridized Probe Cell. Target - single stranded cDNA. Oligonucleotide probe. 5µm.

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1.01k views • 80 slides

Molecular Diagnostics – Quiz 11

Molecular Diagnostics – Quiz 11. Blocking and washing both contribute to the specificity and sensitivity of the 14;18 translocation assay. What else contributes to the specificity ?

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