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Modifying glycosylation Add or subtract sites to your favorite protein (cis) 1a. Subtract sites: Easy, change N or S or

Modifying glycosylation Add or subtract sites to your favorite protein (cis) 1a. Subtract sites: Easy, change N or S or T to A by site-directed mutagenesis 1b. Add sites: Not so easy. Consensus N-X-S does not work, e.g.: requires the insertion of a ~12 aa region encompassing

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