120. 110. 100. 54.8%. 100%. 54.8%. 100.0%. 90. 80. 70. 60. 50. 60.9%. 20.2%. 40. 30. p<.05. 20. *. % of Colonies per 12 High Powered Fields. 10. p<.01. **. 0. + – – –. DMSO/SCRB siRNA. – + – –. DMSO/PEA3 siRNA. – – + –.
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Supplemental Figure 2. Contrl siRNA + - PIK3R3 siRNA - +. A. B. PIK3R3. actin. C. Control siRNA PIK3R3 siRNA. D. Control siRNA. PIK3R3 siRNA. Brdu -FITC. Annexin -V. 2.9%. 2.58%. 7-ADD. PI.
p < 0.01. Nonspecific siRNA Noxa siRNA. Supplemental Figure 4. Noxa mediates synergism by the ABT-737/etoposide combination. UM-22A cells were transfected with 100 nM Noxa siRNA (5’-GUAAUUAUUGACACAUUUCTT-3’)
Supplemental data S1. SW480. Control siRNA BRG1 siRNA. PTEN – Akt – p-Akt – p-GSK-3 b – Cyclin D1 – BRG1 – b -actin –.
Selected siRNA. Control siRNA. A. B. CTSS. CTSS. Selected siRNA. Control siRNA. 1 0.2 . CD137L. CD137L. 1 0.1 . EFNA3. MDK. 1 0.2. 1 0.2 . GDF15. NEO1. 1 0.2 . 1 0.1 . GTF2I. SERPINI1. 1 0.5 . 1 0.2 . MDK. PODXL.
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Supplemental Figure 3. A. B. Ctrl siRNA. PIK3R3 siRNA. Ctrl siRNA + - PIK3R3 siRNA - +. p-RB (ser 780). Cyclin D1. p-AKT (ser 473). p- mTOR (ser 2448). PIK3R3. actin.
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