From rPERK to myc-tagged mPERK: Construction of pShuttle-loxp-tpA-loxp-mPERK_9E10-IRES-hrGFP-1 plasmid for conditional rescued PERK KO mouse. Loxp- tpA -loxp. EcoR V -r PERK - SmaI. A myc-tagged mouse PERK became available. Better to use mouse PERK in mouse system
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Ligand. Receptor-mediated generation of IP 3 and/or activation of voltage-gated channels stimulates ER calcium mobilization and activation of PERK. Ca 2+. GCPR. Endoplasmic reticulum. IP 3. Ca 2+ = 5x10 -5 M. IP 3 R. SERCA. BiP. Ca 2+. RyR. Ca 2+. ATP. Inactive PERK.
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Construction of liver-specific PERK transgenic vector. Why liver specific transgene ?. Alb-aFP-Cre. Kellendonk C, et al. Hepatocyte-specific expression of Cre recombinase. Genesis. 2000;26:151-3. Alb-aFP vector. From Dr. Kellendonk C @ Columbia University.
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Discussion of Compensation regulation and perquisite consumption of SOEs in China (by Donghua Chen, Xinyuan Chen, and Hualin Wan). Qiao Liu, HKU. International Conference on Corporate Governance in Asia and China March 13, 2005.
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