'Polymerase' diaporamas de présentation

Chapter 8 Major Shifts in Prokaryotic Transcription

Chapter 8 Major Shifts in Prokaryotic Transcription.

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MMG /BIOC 352

MMG /BIOC 352. The Replisome: DNA Replication in E. coli and Eukaryotes. Spring 2006. Scott W. Morrical. Contact Information. Scott W. Morrical Given B407 656-8260 Scott.Morrical@uvm.edu. Lecture Outline:. Overview of DNA Replication Bacterial systems ( E. coli)

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????? ???

????? ???. ??? ???. 1.1 ???????? 1.2 ?????????? 1.3 ???????? 1.4 ????????? 1.5 ?????? 1.6 ??????????. ?? (p1-55). 1. ???????? (p1-6) 2. ?????????? (p7-27) 3. ???????? (p28-31) 4. ????????? (p32-42) 5. ?????? (p43-44) 6. ?????????? (p45-53).

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DNA Replication and PCR

DNA Replication and PCR. Central Dogma. DNA structure. nucleotides. Pentose (5 Carbon) sugar. phosphate. At physiological pH phosphate group is charged. ?? What implications does this have for DNA molecules?. Bases. purines. pyrimidines. How are DNA monomers put together.

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AS 90715 Describe the role of DNA in relation to gene expression

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Macromolecular Machines - Introduction

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About how many cells are our bodies made of?

About how many cells are our bodies made of?. TRILLIONS! How many of those cells contain your DNA? All of them! All of your cells contain a complete copy of your genome (DNA)!. Your life started as just 1 cell. How do we come from 1 cell & end up as trillions?.

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DNA Computing

DNA Computing. Herman G. Meyer III Sept. 28, 2004. Overview. DNA DNA/CPU Comparison Leonard M. Adleman Proof of Concept Experiment. DNA. Adenine, Thymine, Guanine, & Cytosine (A,T,C,G) Polymerase Watson-Crick Pairing (A-T,C-G) Cheap Compact Data Storage 1 cm^3 DNA = 10^12 CDs

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MB&B 208: Molecular Biology Fall 2006 MWF, 10-11 Hall-Atwater 84

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replication fork

3'. RNA Primase. 5'. Topoisomerase. Single-strand binding proteins. RNA Primer. replication fork. DNA Helicase. 5'. DNA Polymerase. 3'. leading strand. 5'. direction of DNA Polymerase. Topoisomerase. Single-strand binding proteins. RNA Primer. replication fork. DNA Helicase. 5'.

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DNA Replication

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Polymerase Chain Reactions: Denaturation, annealing & elongation

Polymerase Chain Reactions: Denaturation, annealing & elongation. 95 o C 50-65 o C 72 o C Polymerase is thermophilic can withstand high temperature. Exponential growth. Generally take through 20-30 cycles 2(doubling with every cycle) 20-30(cycles) 2 20 = 10 6 copies 2 30 =10 9 copies.

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D) What is the nitrogen base depicted?__________ ( 2pts )

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Thrust 3: Molecular Propulsion: Chemical Sensing and Chemotaxis of DNA Driven by RNA Polymerase

University of Wisconsin-Madison NSEC: Templated Synthesis and Assembly at the Nanoscale. Thrust 3: Molecular Propulsion: Chemical Sensing and Chemotaxis of DNA Driven by RNA Polymerase Yu, H., K. Jo, K.L. Kounovsky , J.J. de Pablo, and D.C. Schwartz.

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