'Primary antibody' diaporamas de présentation

Presented by: Group 8 @ 23 March 2009

MB 206 Microbial Biotechnology Presentation. Southern, Northern, western Blot. Presented by: Group 8 @ 23 March 2009. OBJECTIVES. * To understand the techniques of molecular searching ( Western, Northern, Southern Blots) * To differentiate the advantages and

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Specialized Prep Techniques

Specialized Prep Techniques. Labels:. To identify the composition of the structure/organelle and/or phase of cycle. Identification of questionable structures. Follow cytochemical pathway through a time course. Localize genetic products, introduced compounds. Cryo-fixation :.

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ELISA Immuno Explorer TM Influenza Diagnostic Tool

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Western blot, Protein electrophoresis, ELISA, Protein chip

Western blot, Protein electrophoresis, ELISA, Protein chip. From DNA to protein. Why it is easy to separate DNA?. The structure of proteins. The classes of amino-acids. 3D structure of protein. The isolation of proteins.

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Binding and Structural Factors that Influence Protein Function And

Binding and Structural Factors that Influence Protein Function And The Application of Protein Function. Chapter 5 (Page 170-182). The Complexity of Hemoglobin Binding O 2 Beyond Subunit Cooperativity. 1. Hemoglobin in Cellular Respiration.

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Immuno-diagnosis

Immuno-diagnosis. Dr. B.S. Vishwanath Professor, Department of Studies in Biochemistry University of Mysore Registrar, Karnataka State Open University Manasagangotri, Mysore. Design of a technique. Physical and chemical properties of a molecule. Size and shape. Ionic charge.

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St. Josephs Cancer Research

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Announcements

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Which plant is transgenic?

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The basics of immunohistochemistry

The basics of immunohistochemistry. principle. Antigen-antibody interactions. Antibodies produced. Antibodies binds antigen. Antigen. HOW IT IS EMPLOYED?. Antigen = Protein of our interest Antibody= we generate . Techniques based on this principle. ELISA FACS WESTERN BLOTTING

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MCB 7200: Molecular Biology

MCB 7200: Molecular Biology. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

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Wavelength and Light Properties

575 nm. Wavelength and Light Properties. (l). High Energy. Low Energy. n = c / l E = h n. Unvisible UV Light. Visible Blue Light. Discovery of the Fluorescence: 1852. Sir George Gabriel Stokes (1819-1903). Fruorite. Fluorescence. - escence - means "to become" in Latin .

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Laboratory procedure that allows you to:

Western Analysis. Laboratory procedure that allows you to:. 1. Verify the expression of a protein. 2. Determine the relative amount of a protein present in different samples. 3. Analyze protein-protein interactions. Two Main Types of Westerns. 1. Denaturing (Most Commonly Used).

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Cell Separation Using Magnetic Beads

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WHAT IS A WESTERN BLOT?

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Immunology Session 2 Structure and production of a ntibodies

Immunology Session 2 Structure and production of a ntibodies. Immunology Tutorial Outline. 1 Antibody structure Role of antibody in defence Polyclonal antibodies Monoclonal antibodies. 1 revision Antibody structure (p295). Antibody structure (p295). Antibody facts.

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Na + /H + exchanger regulatory factor 1 (NHERF1) and angiogenesis in familial breast cancer

FIRENZE 7- 9 Settembre 2009. Na + /H + exchanger regulatory factor 1 (NHERF1) and angiogenesis in familial breast cancer A Mangia*, A Malfettone*, C Salvatore**, B Stea*, G Simone** and A Paradiso* *Clinical Experimental Oncology Laboratory, National Cancer Institute-Bari

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Research Techniques Made Simple: Immunofluorescence Techniques

Research Techniques Made Simple: Immunofluorescence Techniques. Ian D. Odell 1 , MD, PhD and Deborah Cook 2 , MD Departments of Medicine 1 and Pathology 2 University of Vermont College of Medicine Burlington, VT 05405.

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Principle of ECL western blotting

Principle of ECL western blotting. HRP: Horseradish Peroxidase The light peaks 5-20 minutes and decays slowly with a half life of 1 hour. Reagents. TBS: Tris buffered saline TBST: TBS plus Tween 20 (0.1%),

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Supplemental data

Supplemental data. A. ILG+Dima. Dima. Cont. ILG. c-Fos. c-Jun. GAPDH. B. c-Fos. 150. c-Jun. **. 100. Protein Level (% of GAPDH). 50. ***. 0. ILG. cont. Dima. ILG+Dima. Supplemental Data Materials and Method Western Blot

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