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Protein Sequencing Research Group: Results of the PSRG 2011 Study

Protein Sequencing Research Group: Results of the PSRG 2011 Study

Protein Sequencing Research Group: Results of the PSRG 2011 Study. Sensitivity assessment of Edman and Mass Spectrometric Terminal Sequencing of an undisclosed protein. Current PSRG Members. Jim Walters (Chair) Sigma-Aldrich J. Steve Smith University of Texas Medical Branch at Galveston

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905 views • 51 slides
Lab Techniques

Lab Techniques

Lab Techniques. James Chappell & Cheuk Ka Tong. Contents Page. Restriction Enzymes Gel Electrophoresis Blotting techniques-Southern, Northern and Western DNA sequencing Polymerase Chain Reaction (PCR) Recombinant DNA Gene Cloning References/ Recommended Reading. Restriction Enzymes.

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649 views • 31 slides
Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI TOF)

Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI TOF)

Sandipan Ray Suruchi Rao Harini Chandra. Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI TOF).

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914 views • 22 slides
Lab discussion

Lab discussion

Lab discussion. Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) Purification table Paper. SDS-PAGE. Separate proteins according to size Here: actual size, not effective size as for gel filtration/size exclusion Goal: visualization ( typically not a purification step)

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284 views • 16 slides
DSC instrument design and corresponding experimental methodology

DSC instrument design and corresponding experimental methodology

DSC instrument design and corresponding experimental methodology. The technical challenge with DSC. In DSC we are attempting to determine the heat capacity of a biomolecule, in aqueous solution, as a function of temperature

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407 views • 14 slides
Western Blot

Western Blot

Western Blot. Megan Sova Stacie Huffmon Beth Meyer. What is the method?. This is the last step of a long process to be able to identify a specific protein. How is it performed?. Obtain a protein sample Run samples on gel to separate for size (SDS-PAGE) Transfer gel proteins onto membrane

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331 views • 7 slides
Antibody Array Assay Report

Antibody Array Assay Report

Antibody Array Assay Report. Protocol. Protein Extraction Wash the cells with ice cold 1X PBS. Add Lysis Beads and Extraction Buffer to the sample. Mix rigorously by vortexing for 30 seconds. Incubate the mixture on ice for 10 minutes.

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126 views • 4 slides

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